Linked Life Data

1455939

Source:http://linkedlifedata.com/resource/pubmed/id/1455939

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
7
pubmed:dateCreated
1993-1-6
pubmed:abstractText
The unlabelled peroxidase-antiperoxidase (PAP)-technique was compared with the avidin-biotin-peroxidase-complex (ABC)-technique in the identification of Listeria antigen in formalin-fixed paraffin sections of 58 ruminant brains, 44 of which showed histopathological lesions typical for listeric encephalitis. Rabbit hyperimmune serum, obtained by immunization with a Listeria monocytogenes strain of serotype 1/2 a, served as primary serum in the immunohistochemical investigations. The antiserum was tested for specificity using formalin-fixed smears of various bacterial species. Listeria antigen was demonstrated in 40 of the 44 brains with histopathological CNS alterations, as seen in listeriosis, using the ABC method, whereas their identification with the PAP method only succeeded in 34 of the brains. Despite the higher dilution of the primary antibody, the ABC method distinguished itself further, in comparison with the PAP method, through more intense immunohistochemical staining of Listeria antigen. Listeria spp, could be isolated from 46 of 52 brains, which were also examined bacteriologically. They could be isolated from 14 brains, which showed no histopathological lesions indicative of listeriosis. In contrast to this, Listeria antigen was only detected immunohistologically in brains with typical histological listeric CNS alterations. In three cases the presumptive histological diagnosis could not be confirmed immunohistologically.
pubmed:language
ger
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
0514-7166
pubmed:author
pubmed:issnType
Print
pubmed:volume
39
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
473-84
pubmed:dateRevised
2010-11-18
pubmed:meshHeading
pubmed:year
1992
pubmed:articleTitle
[The diagnosis of listeria encephalitis in ruminants using cultural and immunohistologic methods. II. Immunohistologic studies in formalin-fixed paraffin sections].
pubmed:affiliation
Institut für Mikrobiologie und Tierseuchen, Tierärztlichen Hochschule Hannover, Deutschland.
pubmed:publicationType
Journal Article, English Abstract