Linked Life Data

14558773

Source:http://linkedlifedata.com/resource/pubmed/id/14558773

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
22
pubmed:dateCreated
2003-10-15
pubmed:abstractText
Soil organic N accounts for 95-98% of the total soil N content with amino acids (AAs) and amino sugars (ASs) identified as the major soil organic N compounds, but traditional 6 M HCl with reflux or sealed digestions for 24 h and various detection systems have accounted for only 30-40% of soil total N content as AA-N. This study compared traditional HCl extraction methodology with methanesulfonic acid (MSA) hydrolysis and nonderivatized AA and AS quantification by ion chromatography with pulsed amperometric detection for determination of the AA composition of plant litter and soils. MSA (4 M) gave AA-N recovery comparable to or better than 6 M HCl for plant AA digestions (16 h, 121 degrees C, 104 kPa). Use of 4 M MSA (0.5-1.5 h, 136 degrees C, 112 kPa) increased the total recovery of organic N as AAs, ASs, and NH(4)(+) by 46% from soils (n = 22) compared with 6 M HCl (12 h, 110 degrees C, reflux) with a MSA recovery rate of 85.6% of the total N content (n = 22 soils). The shorter MSA soil digestions (0.5-1.5 h) suggested that the majority of soil organic N was not present as protein forms found in plant litter analysis (16 h of digestion). MSA ion chromatographic analysis for soil AA/AS composition is a robust nonderivatization method requiring little sample preparation that can distinguish between small changes in soil AA composition during one growing season due to vegetation and tillage managements.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0021-8561
pubmed:author
pubmed:issnType
Print
pubmed:day
22
pubmed:volume
51
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
6521-9
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
2003
pubmed:articleTitle
Soil amino acid composition quantified by acid hydrolysis and anion chromatography-pulsed amperometry.
pubmed:affiliation
Southwest Watershed Research Center, Agricultural Research Service, U.S. Department of Agriculture, 2000 East Allen Road, Tucson, AZ 85719, USA. dmartens@tucson.ars.ag.gov
pubmed:publicationType
Journal Article, Comparative Study, Research Support, U.S. Gov't, Non-P.H.S.