| pubmed-article:14555204 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:14555204 | lifeskim:mentions | umls-concept:C0019704 | lld:lifeskim |
| pubmed-article:14555204 | lifeskim:mentions | umls-concept:C1622204 | lld:lifeskim |
| pubmed-article:14555204 | lifeskim:mentions | umls-concept:C1159825 | lld:lifeskim |
| pubmed-article:14555204 | pubmed:issue | 8 | lld:pubmed |
| pubmed-article:14555204 | pubmed:dateCreated | 2003-10-13 | lld:pubmed |
| pubmed-article:14555204 | pubmed:abstractText | Cells expressing the envelope glycoprotein complex (Env) encoded by the human immunodeficiency virus can fuse with cells expressing Env receptors (CD4 and CXCR4). The resulting syncytia undergo apoptosis. We developed a cytofluorometric assay for the quantitation of syncytium formation and syncytial apoptosis. Using this methodology, we show that caspase activation in syncytia is inhibited by pharmacological or genetic intervention on cyclin-dependent kinase-1, p53, and mitochondrial membrane permeabilization (MMP). Thus, transfection of fusing cells with the viral mitochondrial inhibitor of apoptosis encoded by cytomegalovirus, a specific inhibitor of MMP, prevented the mitochondrial cytochrome c release and abolished simultaneously the activation of caspase-3. Conversely, inhibition of caspases did not prevent MMP. These results indicate that Env-elicited syncytial apoptosis involves the intrinsic (mitochondrial) pathway. | lld:pubmed |
| pubmed-article:14555204 | pubmed:language | eng | lld:pubmed |
| pubmed-article:14555204 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:14555204 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:14555204 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:14555204 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
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| pubmed-article:14555204 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:14555204 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:14555204 | pubmed:month | Oct | lld:pubmed |
| pubmed-article:14555204 | pubmed:issn | 0006-2952 | lld:pubmed |
| pubmed-article:14555204 | pubmed:author | pubmed-author:PerfettiniJea... | lld:pubmed |
| pubmed-article:14555204 | pubmed:author | pubmed-author:KroemerGuidoG | lld:pubmed |
| pubmed-article:14555204 | pubmed:author | pubmed-author:CastedoMariaM | lld:pubmed |
| pubmed-article:14555204 | pubmed:author | pubmed-author:MétivierDidie... | lld:pubmed |
| pubmed-article:14555204 | pubmed:author | pubmed-author:ZamzamiNaoufa... | lld:pubmed |
| pubmed-article:14555204 | pubmed:author | pubmed-author:RoumierThomas... | lld:pubmed |
| pubmed-article:14555204 | pubmed:author | pubmed-author:AndreauKarine... | lld:pubmed |
| pubmed-article:14555204 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:14555204 | pubmed:day | 15 | lld:pubmed |
| pubmed-article:14555204 | pubmed:volume | 66 | lld:pubmed |
| pubmed-article:14555204 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:14555204 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:14555204 | pubmed:pagination | 1321-9 | lld:pubmed |
| pubmed-article:14555204 | pubmed:dateRevised | 2009-11-19 | lld:pubmed |
| pubmed-article:14555204 | pubmed:meshHeading | pubmed-meshheading:14555204... | lld:pubmed |
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| pubmed-article:14555204 | pubmed:meshHeading | pubmed-meshheading:14555204... | lld:pubmed |
| pubmed-article:14555204 | pubmed:meshHeading | pubmed-meshheading:14555204... | lld:pubmed |
| pubmed-article:14555204 | pubmed:meshHeading | pubmed-meshheading:14555204... | lld:pubmed |
| pubmed-article:14555204 | pubmed:meshHeading | pubmed-meshheading:14555204... | lld:pubmed |
| pubmed-article:14555204 | pubmed:meshHeading | pubmed-meshheading:14555204... | lld:pubmed |
| pubmed-article:14555204 | pubmed:meshHeading | pubmed-meshheading:14555204... | lld:pubmed |
| pubmed-article:14555204 | pubmed:year | 2003 | lld:pubmed |
| pubmed-article:14555204 | pubmed:articleTitle | Mitochondrion-dependent caspase activation by the HIV-1 envelope. | lld:pubmed |
| pubmed-article:14555204 | pubmed:affiliation | Centre National de la Recherche Scientifique, UMR 8125, Institut Gustave Roussy, 39 rue Camille-Desmoulins, F-94805 Villejuif, France. | lld:pubmed |
| pubmed-article:14555204 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:14555204 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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