Linked Life Data

14552825

Source:http://linkedlifedata.com/resource/pubmed/id/14552825

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
2003-10-13
pubmed:abstractText
According to the "middle molecule" (MM) hypothesis, the uremic solutes ranging from 500 to 5,000 Da are insufficiently eliminated by conventional hemodialysis and may act as uremic toxins. However, because of the methodological difficulties of MM purification, their chemical analysis is complicated and the precise structure of these molecules remains obscure. In the present study, a new micro-preparative procedure including SDS electrophoresis and liquid chromatography was applied for isolation of MM peptides from uremic sera. Microsequencing and MS/MS analyses of these peptides showed that most of the identified MM (22 out of 23) represented the N- and C-terminal fragments of the alpha- and beta-chains of fibrinogen. The obtained data provide new information on the precise structure of fibrinogen fragments accumulating in uremic serum as MM.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
1570-0232
pubmed:author
pubmed:issnType
Print
pubmed:day
25
pubmed:volume
796
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
141-53
pubmed:dateRevised
2005-11-17
pubmed:meshHeading
pubmed:year
2003
pubmed:articleTitle
Search for peptidic "middle molecules" in uremic sera: isolation and chemical identification of fibrinogen fragments.
pubmed:affiliation
Heller Institute of Medical Research, Sheba Medical Center, Tel Aviv University, Tel-Hashomer 52621, Israel. kaplanb@sheba.health.gov.il
pubmed:publicationType
Journal Article