Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
23
pubmed:dateCreated
1993-1-7
pubmed:abstractText
Spherical giant liposomes that had encapsulated skeletal-muscle G-actin were made by swelling a dried lipid mixture of dimyristoyl phosphatidylcholine/cardiolipin, 1:1 (wt/wt), in a solution of G-actin/CaCl2 at 0 degree C. Polymerization of the encapsulated G-actin into actin filaments was achieved by raising the temperature to 30 degrees C. We observed the subsequent shape changes of the liposomes by dark-field and differential interference-contrast light microscopy. After approximately 40 min, which was required for completion of actin polymerization, two shapes of liposome were evident: dumbbell and disk. Elongation of the dumbbell-shaped liposomes was concomitant with actin polymerization. Polarization microscopy showed that actin filaments formed thick bundles in the liposomes and that these filaments lay contiguous to the periphery of the liposome. Localization of actin filaments in the liposomes was confirmed by observation of rhodamine phalloidin-conjugated actin filaments by fluorescence microscopy. Both dumbbell- and disk-shaped liposomes were rigid and kept their shapes as far as actin filaments were stabilized. In contrast, liposomes containing bovine serum albumin were fragile, and their shapes continually fluctuated from Brownian motion, indicating that the actin bundles served as mechanical support for the liposome shapes.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/1454846-11221, http://linkedlifedata.com/resource/pubmed/commentcorrection/1454846-1132496, http://linkedlifedata.com/resource/pubmed/commentcorrection/1454846-15398771, http://linkedlifedata.com/resource/pubmed/commentcorrection/1454846-15422102, http://linkedlifedata.com/resource/pubmed/commentcorrection/1454846-2059548, http://linkedlifedata.com/resource/pubmed/commentcorrection/1454846-2067574, http://linkedlifedata.com/resource/pubmed/commentcorrection/1454846-2082725, http://linkedlifedata.com/resource/pubmed/commentcorrection/1454846-2435743, http://linkedlifedata.com/resource/pubmed/commentcorrection/1454846-2548187, http://linkedlifedata.com/resource/pubmed/commentcorrection/1454846-2790010, http://linkedlifedata.com/resource/pubmed/commentcorrection/1454846-2844828, http://linkedlifedata.com/resource/pubmed/commentcorrection/1454846-3294856, http://linkedlifedata.com/resource/pubmed/commentcorrection/1454846-3360852, http://linkedlifedata.com/resource/pubmed/commentcorrection/1454846-37189, http://linkedlifedata.com/resource/pubmed/commentcorrection/1454846-3897278, http://linkedlifedata.com/resource/pubmed/commentcorrection/1454846-4253329, http://linkedlifedata.com/resource/pubmed/commentcorrection/1454846-4254541, http://linkedlifedata.com/resource/pubmed/commentcorrection/1454846-4578441, http://linkedlifedata.com/resource/pubmed/commentcorrection/1454846-4944636, http://linkedlifedata.com/resource/pubmed/commentcorrection/1454846-6548263, http://linkedlifedata.com/resource/pubmed/commentcorrection/1454846-6681986, http://linkedlifedata.com/resource/pubmed/commentcorrection/1454846-6749863, http://linkedlifedata.com/resource/pubmed/commentcorrection/1454846-6826559, http://linkedlifedata.com/resource/pubmed/commentcorrection/1454846-6893482
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0027-8424
pubmed:author
pubmed:issnType
Print
pubmed:day
1
pubmed:volume
89
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
11547-51
pubmed:dateRevised
2011-11-17
pubmed:meshHeading
pubmed:year
1992
pubmed:articleTitle
Morphological changes in liposomes caused by polymerization of encapsulated actin and spontaneous formation of actin bundles.
pubmed:affiliation
Department of Physics, Faculty of Science and Technology, Keio University, Yokohama, Japan.
pubmed:publicationType
Journal Article, In Vitro