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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
23
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pubmed:dateCreated |
1993-1-7
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pubmed:abstractText |
We have constructed a hybrid cDNA coding for a fusion protein between human interleukin 2 and a truncated heavy chain from human immunoglobulin M. The protein encoded by this cDNA contains the entire interleukin 2 sequence including its signal peptide, fused at its C terminus to domains 2 to 4 of the immunoglobulin heavy-chain constant region. Cells transfected with the hybrid cDNA secrete multimeric forms of the fusion protein, which bind specifically to cells bearing high-affinity interleukin 2 receptors. This binding leads either to T-cell proliferation or, if complement is added, to T-cell death. Multimeric forms of the fusion protein with a molecular mass above 500 kDa mediate complement-dependent lysis but trigger proliferation inefficiently when compared with forms with a low molecular mass (< 500 kDa). In contrast, the latter efficiently mediate T-cell proliferation without inducing complement-dependent lysis. The high molecular mass fusion proteins could thus constitute valuable tools for specific immunosuppression in humans.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/1454817-156218,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1454817-1858138,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1454817-1906981,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1454817-1988432,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1454817-1988502,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1454817-2011577,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1454817-2157982,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1454817-2256142,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1454817-2513570,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1454817-2518659,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1454817-2536900,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1454817-2541344,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1454817-2546620,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1454817-2645719,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1454817-2667209,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1454817-2936808,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1454817-3031195,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1454817-3095922,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1454817-3119713,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1454817-3126255,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1454817-3126499,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1454817-3131768,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1454817-3136569,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1454817-3295040,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1454817-3487589,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1454817-3540938,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1454817-3925068,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1454817-5432063,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1454817-6970774
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Dec
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pubmed:issn |
0027-8424
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pubmed:author |
pubmed-author:BonnevilleMM,
pubmed-author:BreathnachRR,
pubmed-author:DietrichJJ,
pubmed-author:GauthierTT,
pubmed-author:GesnelM CMC,
pubmed-author:GodardAA,
pubmed-author:JacquesYY,
pubmed-author:KaraiKK,
pubmed-author:PeyratM AMA,
pubmed-author:SoulillouJ PJP,
pubmed-author:ViéHH
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pubmed:issnType |
Print
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pubmed:day |
1
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pubmed:volume |
89
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
11337-41
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pubmed:dateRevised |
2011-1-27
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pubmed:meshHeading |
pubmed-meshheading:1454817-Base Sequence,
pubmed-meshheading:1454817-Cell Division,
pubmed-meshheading:1454817-Complement System Proteins,
pubmed-meshheading:1454817-Cytotoxicity, Immunologic,
pubmed-meshheading:1454817-DNA,
pubmed-meshheading:1454817-Humans,
pubmed-meshheading:1454817-Immunoglobulin mu-Chains,
pubmed-meshheading:1454817-Interleukin-2,
pubmed-meshheading:1454817-Lymphocyte Activation,
pubmed-meshheading:1454817-Molecular Sequence Data,
pubmed-meshheading:1454817-Oligodeoxyribonucleotides,
pubmed-meshheading:1454817-Polymerase Chain Reaction,
pubmed-meshheading:1454817-Receptors, Interleukin-2,
pubmed-meshheading:1454817-Recombinant Fusion Proteins,
pubmed-meshheading:1454817-Tumor Cells, Cultured
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pubmed:year |
1992
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pubmed:articleTitle |
Human fusion proteins between interleukin 2 and IgM heavy chain are cytotoxic for cells expressing the interleukin 2 receptor.
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pubmed:affiliation |
Institut National de la Santé et de la Recherche Médicale (U.211), Unité de Recherche sur les Effecteurs Lymphocytaires, Nantes, France.
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pubmed:publicationType |
Journal Article,
In Vitro,
Research Support, Non-U.S. Gov't
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