Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
2003-10-9
pubmed:abstractText
In yeast the resistance to kresoxim-methyl and azoxystrobin, like the resistance to strobilurin A (mucidin) is under the control of both mitochondrial cob gene and the PDR network of nuclear genes involved in multidrug resistance. The mucidin-resistant mucl (G137R) and muc2 (L275S) mutants of Saccharomyces cerevisiae containing point mutations in mtDNA were found to be cross-resistant to kresoxim-methyl and azoxystrobin. Cross-resistance to all three strobilurin fungicides was also observed in yeast transformants containing gain-of-function mutations in the nuclear PDR3 gene. On the other hand, nuclear mutants containing disrupted chromosomal copies of the PDR1 and PDR3 genes or the PDR5 gene alone were hypersensitive to kresoxim-methyl, azoxystrobin and strobilurin A. The frequencies of spontaneous mutants selected for resistance either to kresoxim-methyl, azoxystrobin or strobilurin A were similar and resulted from mutations both in mitochondrial and nuclear genes. The results indicate that resistance to strobilurin fungicides, differing in chemical structure and specific activity, can be caused by the same molecular mechanism involving changes in the structure of apocytochrome b and/or increased efflux of strobilurins from fungal cells.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
http://linkedlifedata.com/resource/pubmed/chemical/ATP-Binding Cassette Transporters, http://linkedlifedata.com/resource/pubmed/chemical/Acrylates, http://linkedlifedata.com/resource/pubmed/chemical/Antifungal Agents, http://linkedlifedata.com/resource/pubmed/chemical/DNA-Binding Proteins, http://linkedlifedata.com/resource/pubmed/chemical/Fatty Acids, Unsaturated, http://linkedlifedata.com/resource/pubmed/chemical/Fungal Proteins, http://linkedlifedata.com/resource/pubmed/chemical/Fungicides, Industrial, http://linkedlifedata.com/resource/pubmed/chemical/Methacrylates, http://linkedlifedata.com/resource/pubmed/chemical/PDR1 protein, S cerevisiae, http://linkedlifedata.com/resource/pubmed/chemical/PDR3 protein, S cerevisiae, http://linkedlifedata.com/resource/pubmed/chemical/PDR5 protein, S cerevisiae, http://linkedlifedata.com/resource/pubmed/chemical/Phenylacetates, http://linkedlifedata.com/resource/pubmed/chemical/Pyrimidines, http://linkedlifedata.com/resource/pubmed/chemical/Saccharomyces cerevisiae Proteins, http://linkedlifedata.com/resource/pubmed/chemical/Trans-Activators, http://linkedlifedata.com/resource/pubmed/chemical/Transcription Factors, http://linkedlifedata.com/resource/pubmed/chemical/azoxystrobin, http://linkedlifedata.com/resource/pubmed/chemical/kresoxim-methyl, http://linkedlifedata.com/resource/pubmed/chemical/mucidin
pubmed:status
MEDLINE
pubmed:issn
0015-5632
pubmed:author
pubmed:issnType
Print
pubmed:volume
48
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
496-500
pubmed:dateRevised
2009-11-19
pubmed:meshHeading
pubmed-meshheading:14533481-ATP-Binding Cassette Transporters, pubmed-meshheading:14533481-Acrylates, pubmed-meshheading:14533481-Antifungal Agents, pubmed-meshheading:14533481-Cell Nucleus, pubmed-meshheading:14533481-DNA-Binding Proteins, pubmed-meshheading:14533481-Drug Resistance, Multiple, Fungal, pubmed-meshheading:14533481-Fatty Acids, Unsaturated, pubmed-meshheading:14533481-Fungal Proteins, pubmed-meshheading:14533481-Fungicides, Industrial, pubmed-meshheading:14533481-Genes, Fungal, pubmed-meshheading:14533481-Methacrylates, pubmed-meshheading:14533481-Microbial Sensitivity Tests, pubmed-meshheading:14533481-Mitochondria, pubmed-meshheading:14533481-Mutation, pubmed-meshheading:14533481-Phenylacetates, pubmed-meshheading:14533481-Pyrimidines, pubmed-meshheading:14533481-Saccharomyces cerevisiae, pubmed-meshheading:14533481-Saccharomyces cerevisiae Proteins, pubmed-meshheading:14533481-Trans-Activators, pubmed-meshheading:14533481-Transcription Factors
pubmed:year
2003
pubmed:articleTitle
Cross-resistance to strobilurin fungicides in mitochondrial and nuclear mutants of Saccharomyces cerevisiae.
pubmed:affiliation
Department of Microbiology and Virology, Faculty of Science, Comenius University, 842 15 Bratislava, Slovakia.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't