Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
10
pubmed:dateCreated
2003-10-8
pubmed:abstractText
A 5' nuclease PCR assay for detection of the Yersinia pestis plasminogen activator (pla) gene in human respiratory specimens with simulated Y. pestis infection was developed. An internal positive control was added to the reaction mixture in order to detect the presence of PCR inhibitors that are often found in biological samples. The assay was 100% specific for Y. pestis. In the absence of inhibitors, a sensitivity of 10(2) CFU/ml of respiratory fluid was obtained. When inhibitors were present, detection of Y. pestis DNA required a longer sample treatment time and an initial concentration of bacteria of at least 10(4) CFU/ml. The test's total turnaround time was less than 5 h. The assay described here is well suited to the rapid diagnosis of pneumonic plague, the form of plague most likely to result from a bioterrorist attack.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/14532247-10325359, http://linkedlifedata.com/resource/pubmed/commentcorrection/14532247-10799272, http://linkedlifedata.com/resource/pubmed/commentcorrection/14532247-11472963, http://linkedlifedata.com/resource/pubmed/commentcorrection/14532247-11586360, http://linkedlifedata.com/resource/pubmed/commentcorrection/14532247-2843470, http://linkedlifedata.com/resource/pubmed/commentcorrection/14532247-8157543, http://linkedlifedata.com/resource/pubmed/commentcorrection/14532247-8314993, http://linkedlifedata.com/resource/pubmed/commentcorrection/14532247-8458980, http://linkedlifedata.com/resource/pubmed/commentcorrection/14532247-8981352, http://linkedlifedata.com/resource/pubmed/commentcorrection/14532247-9666006, http://linkedlifedata.com/resource/pubmed/commentcorrection/14532247-9705392
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0095-1137
pubmed:author
pubmed:issnType
Print
pubmed:volume
41
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
4873-5
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
2003
pubmed:articleTitle
Detection of Yersinia pestis in sputum by real-time PCR.
pubmed:affiliation
Laboratoire de Bactériologie-Hygiène, Centre Hospitalier Régional Universitaire de Lille, Lille, France.
pubmed:publicationType
Journal Article, Evaluation Studies