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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
11
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pubmed:dateCreated |
2003-10-8
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pubmed:abstractText |
Cyclooxygenase-2 (COX-2) represents the inducible key enzyme of arachidonic acid metabolism and contributes to the pathogenesis of gastroduodenal ulcers and gastric cancer. Helicobacter pylori infection is associated with elevated gastric COX-2 levels, but the mechanisms underlying H. pylori-dependent cox-2 gene expression are unclear. H. pylori stimulated cox-2 mRNA and protein abundance in gastric epithelial cells in vitro and in vivo, and functional analysis of the cox-2 gene promoter mapped its H. pylori-responsive region to a proximal CRE/Ebox element at -56 to -48. Moreover, USF1/-2 and CREB transcription factors binding to this site were identified to transmit H. pylori-dependent cox-2 transcription. Activation of MEK/ERK1/-2 signalling by bacterial virulence factors located outside the H. pylori cag pathogenicity island (cagPAI) was found to mediate bacterial effects on the cox-2 promoter. Our study provides a detailed description of the molecular pathways underlying H. pylori-dependent cox-2 gene expression in gastric epithelial cells, and may thus contribute to a better understanding of mechanisms underlying H. pylori pathogenicity.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Cyclic AMP Response...,
http://linkedlifedata.com/resource/pubmed/chemical/Cyclooxygenase 2,
http://linkedlifedata.com/resource/pubmed/chemical/DNA-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Isoenzymes,
http://linkedlifedata.com/resource/pubmed/chemical/MAP Kinase Kinase Kinases,
http://linkedlifedata.com/resource/pubmed/chemical/Mitogen-Activated Protein Kinases,
http://linkedlifedata.com/resource/pubmed/chemical/Prostaglandin-Endoperoxide Synthases,
http://linkedlifedata.com/resource/pubmed/chemical/Transcription Factors,
http://linkedlifedata.com/resource/pubmed/chemical/Upstream Stimulatory Factors,
http://linkedlifedata.com/resource/pubmed/chemical/Usf1 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Virulence Factors
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pubmed:status |
MEDLINE
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pubmed:month |
Nov
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pubmed:issn |
1462-5814
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pubmed:author |
pubmed-author:CramerThorstenT,
pubmed-author:FischerSusan MSM,
pubmed-author:GaoFengF,
pubmed-author:HöckerMichaelM,
pubmed-author:JüttnerStefanS,
pubmed-author:MeyerThomas FTF,
pubmed-author:NaumannMichaelM,
pubmed-author:SchmidtWolfgang EWE,
pubmed-author:SchmitzFrankF,
pubmed-author:WalduckAnnaA,
pubmed-author:WeberMatthiasM,
pubmed-author:WesslerSiljaS,
pubmed-author:WiedenmannBertramB,
pubmed-author:WunderChristianC
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pubmed:issnType |
Print
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pubmed:volume |
5
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
821-34
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:14531897-Animals,
pubmed-meshheading:14531897-Cyclic AMP Response Element-Binding Protein,
pubmed-meshheading:14531897-Cyclooxygenase 2,
pubmed-meshheading:14531897-DNA-Binding Proteins,
pubmed-meshheading:14531897-Epithelial Cells,
pubmed-meshheading:14531897-Gastric Mucosa,
pubmed-meshheading:14531897-Gene Expression Regulation,
pubmed-meshheading:14531897-Helicobacter Infections,
pubmed-meshheading:14531897-Helicobacter pylori,
pubmed-meshheading:14531897-Isoenzymes,
pubmed-meshheading:14531897-MAP Kinase Kinase Kinases,
pubmed-meshheading:14531897-MAP Kinase Signaling System,
pubmed-meshheading:14531897-Mice,
pubmed-meshheading:14531897-Mitogen-Activated Protein Kinases,
pubmed-meshheading:14531897-Promoter Regions, Genetic,
pubmed-meshheading:14531897-Prostaglandin-Endoperoxide Synthases,
pubmed-meshheading:14531897-Transcription, Genetic,
pubmed-meshheading:14531897-Transcription Factors,
pubmed-meshheading:14531897-Upstream Stimulatory Factors,
pubmed-meshheading:14531897-Virulence Factors
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pubmed:year |
2003
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pubmed:articleTitle |
Helicobacter pylori stimulates host cyclooxygenase-2 gene transcription: critical importance of MEK/ERK-dependent activation of USF1/-2 and CREB transcription factors.
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pubmed:affiliation |
Medizinische Klinik mit Schwerpunkt Hepatologie und Gastroenterologie, Charité- Universitätsmedizin Berlin, Campus Virchow-Klinikum, Augustenburger Platz 1, 13353 Berlin, Germany.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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