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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
52
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pubmed:dateCreated |
2003-12-22
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pubmed:abstractText |
Depletion of intracellular Ca2+ stores leads to the activation of Ca2+ inflow through store-operated Ca2+ channels. Although the identity of these channels is unknown, there is considerable evidence that the transient receptor potential channel 1 (TRPC1) participates in the formation of these channels. We show that TRPC1 physically interacts with the a-isoform of the inhibitor of the myogenic family (I-mfa), a known inhibitor of basic helix-loop-helix transcription factors, in vitro and in vivo. The interaction is mediated by the C-terminal cytoplasmic tail of TRPC1 and the C-terminal cysteine-rich domain of I-mfa. Using the whole cell configuration of the patch clamp technique, we show that ectopic expression of I-mfa in CHO-K1 cells reduces native store-activated Ca2+ currents, whereas knock-down of endogenous I-mfa in A431 cells by RNA interference enhances these currents. Pipette perfusion of purified recombinant I-mfa rescues the effect of I-mfa knock-down on store-operated conductance. Finally, cell dialysis with a monoclonal antibody specific to TRPC1 results in the suppression of store-activated conductance in cells lacking I-mfa, but not in I-mfa expressing cells. We propose that I-mfa functions as a molecular switch to suppress the store dependence of TRPC1.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antibodies, Monoclonal,
http://linkedlifedata.com/resource/pubmed/chemical/Calcium,
http://linkedlifedata.com/resource/pubmed/chemical/Calcium Channels,
http://linkedlifedata.com/resource/pubmed/chemical/Cysteine,
http://linkedlifedata.com/resource/pubmed/chemical/MDFI protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/MYOG protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Mdfi protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Myog protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Myog protein, rat,
http://linkedlifedata.com/resource/pubmed/chemical/Myogenic Regulatory Factors,
http://linkedlifedata.com/resource/pubmed/chemical/Myogenin,
http://linkedlifedata.com/resource/pubmed/chemical/Protein Isoforms,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/TRPC Cation Channels,
http://linkedlifedata.com/resource/pubmed/chemical/Transcription Factors,
http://linkedlifedata.com/resource/pubmed/chemical/transient receptor potential...
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pubmed:status |
MEDLINE
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pubmed:month |
Dec
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pubmed:issn |
0021-9258
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
26
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pubmed:volume |
278
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
52763-72
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pubmed:dateRevised |
2007-11-14
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pubmed:meshHeading |
pubmed-meshheading:14530267-Animals,
pubmed-meshheading:14530267-Antibodies, Monoclonal,
pubmed-meshheading:14530267-CHO Cells,
pubmed-meshheading:14530267-Calcium,
pubmed-meshheading:14530267-Calcium Channels,
pubmed-meshheading:14530267-Cell Line,
pubmed-meshheading:14530267-Cricetinae,
pubmed-meshheading:14530267-Cysteine,
pubmed-meshheading:14530267-Cytoplasm,
pubmed-meshheading:14530267-Electrophysiology,
pubmed-meshheading:14530267-Female,
pubmed-meshheading:14530267-Humans,
pubmed-meshheading:14530267-Kidney,
pubmed-meshheading:14530267-Liver,
pubmed-meshheading:14530267-Mice,
pubmed-meshheading:14530267-Mice, Inbred BALB C,
pubmed-meshheading:14530267-Myogenic Regulatory Factors,
pubmed-meshheading:14530267-Myogenin,
pubmed-meshheading:14530267-Plasmids,
pubmed-meshheading:14530267-Precipitin Tests,
pubmed-meshheading:14530267-Protein Binding,
pubmed-meshheading:14530267-Protein Isoforms,
pubmed-meshheading:14530267-Protein Structure, Tertiary,
pubmed-meshheading:14530267-RNA Interference,
pubmed-meshheading:14530267-Rats,
pubmed-meshheading:14530267-Recombinant Proteins,
pubmed-meshheading:14530267-TRPC Cation Channels,
pubmed-meshheading:14530267-Transcription Factors,
pubmed-meshheading:14530267-Transfection,
pubmed-meshheading:14530267-Two-Hybrid System Techniques
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pubmed:year |
2003
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pubmed:articleTitle |
Inhibitor of myogenic family, a novel suppressor of store-operated currents through an interaction with TRPC1.
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pubmed:affiliation |
Department of Cell Biology, University of Oklahoma Health Sciences Center, Oklahoma City, Oklahoma 73104, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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