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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
40
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pubmed:dateCreated |
2003-10-7
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pubmed:databankReference |
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pubmed:abstractText |
Sulerythrin is the first rubrerythrin-like protein to be isolated from an aerobic organism, Sulfolobus tokodaii strain 7, and it lacks a C-terminal rubredoxin-like FeS(4) domain. The protein purified from Sulfolobus cells was crystallized, and the crystal structure was determined at 1.7 A resolution. The dimer of sulerythrin exhibited "domain-swapping" at the loop connecting alphaB and alphaC, hybrid four-helix bundles consisting of alphaA/B and alphaC/D being formed. The structure and atomic identity of the binuclear metal center were determined by means of anomalous scattering analysis. The site contained 1.0 mol of hexacoordinate Fe, 0.80-0.87 mol of tetracoordinate Zn, and 0.73-0.88 mol of putative O(2) per monomer. The metal ions were found at exchanged positions compared to those in the Fe/Zn-containing rubrerythrin from Desulfovibrio vulgaris. The results demonstrate that the binuclear metal center of rubrerythrin-like proteins is plastic in its ability to bind metal ions.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
0006-2960
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
14
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pubmed:volume |
42
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
11707-15
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:14529281-Aerobiosis,
pubmed-meshheading:14529281-Amino Acid Sequence,
pubmed-meshheading:14529281-Archaeal Proteins,
pubmed-meshheading:14529281-Bacterial Proteins,
pubmed-meshheading:14529281-Binding Sites,
pubmed-meshheading:14529281-Computer Simulation,
pubmed-meshheading:14529281-Crystallization,
pubmed-meshheading:14529281-Crystallography, X-Ray,
pubmed-meshheading:14529281-Dimerization,
pubmed-meshheading:14529281-Ferredoxins,
pubmed-meshheading:14529281-Hemerythrin,
pubmed-meshheading:14529281-Iron,
pubmed-meshheading:14529281-Models, Molecular,
pubmed-meshheading:14529281-Molecular Sequence Data,
pubmed-meshheading:14529281-Protein Structure, Tertiary,
pubmed-meshheading:14529281-Rubredoxins,
pubmed-meshheading:14529281-Sequence Homology, Amino Acid,
pubmed-meshheading:14529281-Sulfolobus,
pubmed-meshheading:14529281-Zinc
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pubmed:year |
2003
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pubmed:articleTitle |
Crystal structure of sulerythrin, a rubrerythrin-like protein from a strictly aerobic archaeon, Sulfolobus tokodaii strain 7, shows unexpected domain swapping.
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pubmed:affiliation |
Department of Biotechnology, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan. asfushi@mail.ecc.u-tokyo.ac.jp
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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