Source:http://linkedlifedata.com/resource/pubmed/id/14527718
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:dateCreated |
2003-10-6
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| pubmed:abstractText |
Flap endonuclease-1 (FEN-1) is an important enzyme involved in DNA replication and repair. Previously, we isolated and characterized a complementary DNA (cDNA) from rice (Oryza sativa) encoding a protein which shows homology with the eukaryotic flap endonuclease-1 (FEN-1). In this report, we found that rice (O. sativa L. cv. Nipponbare) possessed two FEN-1 homologues designated as OsFEN-1a and OsFEN-1b. The OsFEN-1a and OsFEN-1b genes were mapped to chromosome 5 and 3, respectively. Both genes contained 17 exons and 16 introns. Alignment of OsFEN-1a protein with OsFEN-1b protein showed a high degree of sequence similarity, particularly around the N and I domains. Northern hybridization and in situ hybridization analysis demonstrated preferential expression of OsFEN-1a and OsFEN-1b in proliferating tissues such as the shoot apical meristem or young leaves. The levels of OsFEN-1a and OsFEN-1b expression were significantly reduced when cell proliferation was temporarily halted by the removal of sucrose from the growth medium. When the growth-halted cells began to regrow following the addition of sucrose to the medium, both OsFEN-1a and OsFEN-1b were again expressed at high level. These results suggested that OsFEN-1a and OsFEN-1b are required for cell proliferation. Functional complementation assay suggested that OsFEN-1a cDNA had the ability to complement Saccharomyces cerevisiae rad27 null mutant. On the other hand, OsFEN-1b cDNA could not complement the rad27 mutant. The roles of OsFEN-1a and OsFEN-1b in plant DNA replication and repair are discussed.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical | |
| pubmed:status |
MEDLINE
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| pubmed:month |
Sep
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| pubmed:issn |
0378-1119
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| pubmed:author | |
| pubmed:issnType |
Print
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| pubmed:day |
18
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| pubmed:volume |
314
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
63-71
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| pubmed:dateRevised |
2006-11-15
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| pubmed:meshHeading |
pubmed-meshheading:14527718-Amino Acid Sequence,
pubmed-meshheading:14527718-Cell Division,
pubmed-meshheading:14527718-Chromosome Mapping,
pubmed-meshheading:14527718-Chromosomes, Plant,
pubmed-meshheading:14527718-Cloning, Molecular,
pubmed-meshheading:14527718-Exons,
pubmed-meshheading:14527718-Flap Endonucleases,
pubmed-meshheading:14527718-Gene Expression Regulation, Developmental,
pubmed-meshheading:14527718-Gene Expression Regulation, Enzymologic,
pubmed-meshheading:14527718-Gene Expression Regulation, Plant,
pubmed-meshheading:14527718-Genes, Plant,
pubmed-meshheading:14527718-Genetic Complementation Test,
pubmed-meshheading:14527718-In Situ Hybridization,
pubmed-meshheading:14527718-Introns,
pubmed-meshheading:14527718-Isoenzymes,
pubmed-meshheading:14527718-Molecular Sequence Data,
pubmed-meshheading:14527718-Mutation,
pubmed-meshheading:14527718-Oryza sativa,
pubmed-meshheading:14527718-Phylogeny,
pubmed-meshheading:14527718-Plant Proteins,
pubmed-meshheading:14527718-Protein Conformation,
pubmed-meshheading:14527718-Saccharomyces cerevisiae,
pubmed-meshheading:14527718-Sequence Alignment,
pubmed-meshheading:14527718-Sequence Homology, Amino Acid
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| pubmed:year |
2003
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| pubmed:articleTitle |
Functional characterization of two flap endonuclease-1 homologues in rice.
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| pubmed:affiliation |
Department of Applied Biological Science, Faculty of Science and Technology, Tokyo University of Science, 2641 Yamazaki, Chiba, 278-8510, Noda, Japan.
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| pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, Non-U.S. Gov't
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