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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
11
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pubmed:dateCreated |
1993-1-6
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pubmed:abstractText |
Approximately 10% of the world population is infected with Entamoeba histolytica, but only 10% of the carriers develop symptomatic amebiasis. This discrepancy could be explained by the genotypic differences between the morphologically indistinguishable invasive and noninvasive strains of E. histolytica currently identified by zymodeme analysis, a technique that is unsuitable for routine diagnostic laboratories. Here we report the production of a monoclonal antibody against E. histolytica and its use in an immunofluorescence assay to identify invasive isolates cultured from stool samples of infected patients in several regions where amebiasis is endemic: Bangladesh, Colombia, and Mexico. After testing a total of 88 E. histolytica isolates, the correlation between zymodeme characterization and the immunofluorescence assay with the invasive isolate-specific monoclonal antibody was 100%. The epitope detected by the invasive isolate-specific monoclonal antibody resides in a previously undescribed internal protein with molecular masses of 84 and 81 kDa in axenic and polyxenic E. histolytica strains, respectively.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/1452651-111249,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1452651-13139297,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1452651-15395136,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1452651-1566286,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1452651-1566287,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1452651-1594290,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1452651-1685555,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1452651-1692809,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1452651-1705935,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1452651-1825198,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1452651-1939576,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1452651-206991,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1452651-214906,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1452651-2150165,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1452651-2174899,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1452651-2180826,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1452651-2205653,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1452651-2278073,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1452651-2332466,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1452651-2537259,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1452651-2544890,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1452651-2556432,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1452651-2558434,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1452651-2885384,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1452651-2894495,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1452651-388439,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1452651-3981000,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1452651-4296113,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1452651-4355103,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1452651-5432063,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1452651-6194223,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1452651-6207242,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1452651-6255635,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1452651-6259781,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1452651-6306876,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1452651-7009747,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1452651-714156,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1452651-7300683
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Nov
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pubmed:issn |
0095-1137
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
30
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pubmed:owner |
NLM
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pubmed:authorsComplete |
N
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pubmed:pagination |
2807-13
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pubmed:dateRevised |
2010-9-7
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pubmed:meshHeading |
pubmed-meshheading:1452651-Animals,
pubmed-meshheading:1452651-Antibodies, Monoclonal,
pubmed-meshheading:1452651-Antibodies, Protozoan,
pubmed-meshheading:1452651-Antigens, Protozoan,
pubmed-meshheading:1452651-Carrier State,
pubmed-meshheading:1452651-Entamoeba histolytica,
pubmed-meshheading:1452651-Entamoebiasis,
pubmed-meshheading:1452651-Evaluation Studies as Topic,
pubmed-meshheading:1452651-Humans,
pubmed-meshheading:1452651-Mice,
pubmed-meshheading:1452651-Virulence
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pubmed:year |
1992
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pubmed:articleTitle |
A monoclonal antibody for distinction of invasive and noninvasive clinical isolates of Entamoeba histolytica.
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pubmed:affiliation |
Department of Medical Parasitology, London School of Hygiene and Tropical Medicine, United Kingdom.
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pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, Non-U.S. Gov't
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