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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
20
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pubmed:dateCreated |
2003-10-3
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pubmed:abstractText |
Escherichia coli responses to four inhibitors that interfere with translation were monitored at the transcriptional level. A DNA microarray method provided a comprehensive view of changes in mRNA levels after exposure to these agents. Real-time reverse transcriptase PCRanalysis served to verify observations made with microarrays, and a chromosomal grpE::lux operon fusion was employed to specifically monitor the heat shock response. 4-Azaleucine, a competitive inhibitor of leucyl-tRNA synthetase, surprisingly triggered the heat shock response. Administration of mupirocin, an inhibitor of isoleucyl-tRNA synthetase activity, resulted in changes reminiscent of the stringent response. Treatment with kasugamycin and puromycin (targeting ribosomal subunit association as well as its peptidyl-transferase activity) caused accumulation of mRNAs from ribosomal protein operons. Abundant biosynthetic transcripts were often significantly diminished after treatment with any of these agents. Exposure of a relA strain to mupirocin resulted in accumulation of ribosomal protein operon transcripts. However, the relA strain's response to the other inhibitors was quite similar to that of the wild-type strain.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/14526028-10762266,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14526028-1105582,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14526028-11125053,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14526028-11133948,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14526028-11162084,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14526028-11162085,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14526028-11226277,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14526028-11244055,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14526028-11244066,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14526028-11344143,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14526028-11544210,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14526028-12123445,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14526028-12142487,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14526028-12374860,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14526028-12486074,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14526028-2005134,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14526028-2046546,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14526028-3031008,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14526028-3318818,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14526028-3886165,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14526028-4562599,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14526028-4931565,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14526028-6258580,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14526028-6378902,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14526028-7929087,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14526028-8017928,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14526028-8389880,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14526028-8526529,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14526028-9278503,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14526028-9473030,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14526028-9680611
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
0021-9193
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
185
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
6158-70
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:14526028-Aminoglycosides,
pubmed-meshheading:14526028-Anti-Bacterial Agents,
pubmed-meshheading:14526028-Escherichia coli,
pubmed-meshheading:14526028-Escherichia coli Proteins,
pubmed-meshheading:14526028-Gene Expression Profiling,
pubmed-meshheading:14526028-Gene Expression Regulation, Bacterial,
pubmed-meshheading:14526028-Leucine,
pubmed-meshheading:14526028-Mupirocin,
pubmed-meshheading:14526028-Oligonucleotide Array Sequence Analysis,
pubmed-meshheading:14526028-Protein Biosynthesis,
pubmed-meshheading:14526028-Protein Synthesis Inhibitors,
pubmed-meshheading:14526028-Puromycin,
pubmed-meshheading:14526028-Transcription, Genetic
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pubmed:year |
2003
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pubmed:articleTitle |
Interfering with different steps of protein synthesis explored by transcriptional profiling of Escherichia coli K-12.
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pubmed:affiliation |
Central Research and Development, DuPont Company, Wilmington, Delaware 19880-0173, USA
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, U.S. Gov't, Non-P.H.S.
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