Source:http://linkedlifedata.com/resource/pubmed/id/14525783
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
3
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| pubmed:dateCreated |
2004-1-19
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| pubmed:abstractText |
Eliminating alloreactive cells from T-cell populations would enable the transfer of immune function to patients who receive stem cell transplants. However, high-efficiency depletion has proved difficult to achieve. We sought to develop ex vivo approaches for the maximal depletion of alloreactive CD4(+) T cells. Using a flow cytometric cell sorting approach after mixed lymphocyte reaction (MLR) culture, we have found that sorted CFSE(bright) (5-(and-6)-carboxyfluorescein diacetate succinmidyl ester) (nondivided) and activation antigen-negative cells are markedly depleted of alloreactivity. With HLA-mismatched peripheral blood mononuclear cell (PBMC) stimulators we have consistently attained (90%-95%) depletion of alloreactivity. Importantly, when purified matured monocyte-derived dendritic cells (DCs) are used as stimulators, a 100-fold (99%) reduction in alloreactivity was attained, resulting in abrogation of the secondary MLR. Significantly, the CFSE(bright) CD25(-) cells recovered from these cultures retained general immunoreactivity, including responses to Candida and cytomegalovirus (CMV) antigens. In addition, a CFSE-based approach was tested and found to be sufficient for graft-versus-host disease (GVHD) prevention in vivo, in a major histocompatibility complex (MHC) class II disparate murine model. This efficient approach to selectively deplete mature alloantigen-specific T cells may permit enhanced immune reconstitution without GVHD.
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| pubmed:grant | |
| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
AIM
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| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/5-(6)-carboxyfluorescein diacetate...,
http://linkedlifedata.com/resource/pubmed/chemical/Fluoresceins,
http://linkedlifedata.com/resource/pubmed/chemical/Fluorescent Dyes,
http://linkedlifedata.com/resource/pubmed/chemical/HLA Antigens,
http://linkedlifedata.com/resource/pubmed/chemical/Isoantigens,
http://linkedlifedata.com/resource/pubmed/chemical/Succinimides
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| pubmed:status |
MEDLINE
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| pubmed:month |
Feb
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| pubmed:issn |
0006-4971
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| pubmed:author | |
| pubmed:issnType |
Print
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| pubmed:day |
1
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| pubmed:volume |
103
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
1158-65
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| pubmed:dateRevised |
2007-11-14
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| pubmed:meshHeading |
pubmed-meshheading:14525783-Animals,
pubmed-meshheading:14525783-CD4-Positive T-Lymphocytes,
pubmed-meshheading:14525783-Cell Separation,
pubmed-meshheading:14525783-Dendritic Cells,
pubmed-meshheading:14525783-Flow Cytometry,
pubmed-meshheading:14525783-Fluoresceins,
pubmed-meshheading:14525783-Fluorescent Dyes,
pubmed-meshheading:14525783-Graft vs Host Disease,
pubmed-meshheading:14525783-HLA Antigens,
pubmed-meshheading:14525783-Hematopoietic Stem Cell Transplantation,
pubmed-meshheading:14525783-Humans,
pubmed-meshheading:14525783-Isoantigens,
pubmed-meshheading:14525783-Lymphocyte Culture Test, Mixed,
pubmed-meshheading:14525783-Lymphocyte Depletion,
pubmed-meshheading:14525783-Mice,
pubmed-meshheading:14525783-Mice, Inbred C57BL,
pubmed-meshheading:14525783-Succinimides,
pubmed-meshheading:14525783-Transplantation, Homologous
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| pubmed:year |
2004
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| pubmed:articleTitle |
Ex vivo depletion of alloreactive cells based on CFSE dye dilution, activation antigen selection, and dendritic cell stimulation.
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| pubmed:affiliation |
Department of Pediatrics, University of Minnesota Cancer Center, Minneapolis, MN 55455, USA. godfr007@umn.edu
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| pubmed:publicationType |
Journal Article,
In Vitro,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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