Source:http://linkedlifedata.com/resource/pubmed/id/14525773
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
3
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| pubmed:dateCreated |
2004-1-19
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| pubmed:abstractText |
Interleukin-5 (IL-5) is a hematopoietic differentiation factor that promotes the development of mature eosinophils from progenitors in bone marrow. We present a multifactorial microarray study documenting the transcriptional events in bone marrow of wild-type and IL-5-deficient mice at baseline and in response to infection with Schistosoma mansoni. The microarray data were analyzed by a 4-way subtractive algorithm that eliminated confounding non-IL-5-related sequelae of schistosome infection as well as alterations in gene expression among uninfected mice. Among the most prominent findings, we observed 7- to 40-fold increased expression of transcripts encoding the classic eosinophil granule proteins (eosinophil peroxidase, major basic protein, the ribonucleases) together with arachidonate-15-lipoxygenase and protease inhibitor plasminogen activator inhibitor 2 (PAI-2), in the IL-5-producing, infected wild-type mice only. This was accompanied by increased transcription of genes involved in secretory protein biosynthesis and granule-vesicle formation. Interestingly, we did not detect increased expression of genes encoding eosinophil-related chemokine receptors (CCR1, CCR3) or members of the GATA or CCAAT/enhancer binding protein (C/EBP) transcription factor families. These data suggest that the IL-5-responsive progenitors in the mouse bone marrow are already significantly committed to the eosinophil lineage and that IL-5 promotes differentiation of these committed progenitors into cells with recognizable and characteristic cytoplasmic granules and granule proteins.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
AIM
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| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Blood Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/DNA, Complementary,
http://linkedlifedata.com/resource/pubmed/chemical/Eosinophil Granule Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-5,
http://linkedlifedata.com/resource/pubmed/chemical/Ribonucleases
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| pubmed:status |
MEDLINE
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| pubmed:month |
Feb
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| pubmed:issn |
0006-4971
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| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
1
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| pubmed:volume |
103
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| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
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| pubmed:pagination |
868-77
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| pubmed:dateRevised |
2006-11-15
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| pubmed:meshHeading |
pubmed-meshheading:14525773-Animals,
pubmed-meshheading:14525773-Base Sequence,
pubmed-meshheading:14525773-Blood Proteins,
pubmed-meshheading:14525773-DNA, Complementary,
pubmed-meshheading:14525773-Eosinophil Granule Proteins,
pubmed-meshheading:14525773-Eosinophilia,
pubmed-meshheading:14525773-Gene Expression Profiling,
pubmed-meshheading:14525773-Hematopoiesis,
pubmed-meshheading:14525773-Hematopoietic Stem Cells,
pubmed-meshheading:14525773-Interleukin-5,
pubmed-meshheading:14525773-Mice,
pubmed-meshheading:14525773-Mice, Inbred C57BL,
pubmed-meshheading:14525773-Mice, Knockout,
pubmed-meshheading:14525773-Oligonucleotide Array Sequence Analysis,
pubmed-meshheading:14525773-Reverse Transcriptase Polymerase Chain Reaction,
pubmed-meshheading:14525773-Ribonucleases,
pubmed-meshheading:14525773-Schistosomiasis mansoni,
pubmed-meshheading:14525773-Transcription, Genetic
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| pubmed:year |
2004
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| pubmed:articleTitle |
Gene microarray analysis reveals interleukin-5-dependent transcriptional targets in mouse bone marrow.
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| pubmed:affiliation |
NIAID, NIH, Bldg 10, Rm 11N104, 9000 Rockville Pike, Bethesda, MD, 20892, USA. jbystrom@niaid.nih.gov
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| pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
|