Linked Life Data

14522919

Source:http://linkedlifedata.com/resource/pubmed/id/14522919

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
18
pubmed:dateCreated
2003-10-2
pubmed:abstractText
Peroxisome proliferator-activated receptor gamma (PPARgamma) is a member of the nuclear hormonal receptor superfamily expressed in a large number of human cancers. Here, we demonstrate that PPARgamma is expressed and transcriptionally active in breast cancer cells independent of their p53, estrogen receptor, or human epidermal growth factor receptor 2 status. 2-Cyano-3,12-dioxooleana-1,9-dien-28-oic acid (CDDO), a novel synthetic triterpenoid, is a ligand for PPARgamma. We investigated the molecular mechanisms of CDDO on proliferation and apoptosis in breast cancer cells. In all breast cancer cell lines studied, CDDO transactivated PPARgamma, induced dose- and time-dependent cell growth inhibition, cell cycle arrest in G(1)-S and G(2)-M, and apoptosis. We then used differential cDNA array analysis to investigate the molecular changes induced by CDDO. After 16-h exposure of MCF-7 and MDA-MB-435 cells to CDDO, we found genes encoding the following proteins to be up-regulated in both cell lines: p21(Waf1/CIP1); GADD153; CAAT/enhancer binding protein transcription factor family members; and proteins involved in the ubiquitin-proteasome pathway. Among the down-regulated genes, we focused on the genes encoding cyclin D1, proliferating cell nuclear antigen, and the insulin receptor substrate 1. Using Western blot analysis and/or real-time PCR, we confirmed that CDDO regulated the expression of cyclin D1, p21(Waf1/CIP1), and Bcl-2. Cyclin D1 and p21(Waf1/CIP1) were additionally confirmed as important mediators of CDDO growth inhibition in genetically modified breast cancer cell lines. CDDO was able to significantly reduce the growth of MDA-MB-435 tumor cells in immunodeficient mice in vivo. The finding that CDDO can target genes critical for the regulation of cell cycle, apoptosis, and breast carcinogenesis suggests usage of CDDO as novel targeted therapy in breast cancer.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
0008-5472
pubmed:author
pubmed:issnType
Print
pubmed:day
15
pubmed:volume
63
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
5926-39
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed-meshheading:14522919-Animals, pubmed-meshheading:14522919-Apoptosis, pubmed-meshheading:14522919-Breast Neoplasms, pubmed-meshheading:14522919-Cell Differentiation, pubmed-meshheading:14522919-Cell Division, pubmed-meshheading:14522919-Cell Line, Tumor, pubmed-meshheading:14522919-Cyclin D1, pubmed-meshheading:14522919-Cyclin-Dependent Kinase Inhibitor p21, pubmed-meshheading:14522919-Cyclins, pubmed-meshheading:14522919-Down-Regulation, pubmed-meshheading:14522919-Female, pubmed-meshheading:14522919-Gene Expression Regulation, Neoplastic, pubmed-meshheading:14522919-Humans, pubmed-meshheading:14522919-Immunocompromised Host, pubmed-meshheading:14522919-Mice, pubmed-meshheading:14522919-Mice, Nude, pubmed-meshheading:14522919-Oleanolic Acid, pubmed-meshheading:14522919-Receptors, Cytoplasmic and Nuclear, pubmed-meshheading:14522919-Transcription Factors
pubmed:year
2003
pubmed:articleTitle
Activation of peroxisome proliferator-activated receptor gamma by a novel synthetic triterpenoid 2-cyano-3,12-dioxooleana-1,9-dien-28-oic acid induces growth arrest and apoptosis in breast cancer cells.
pubmed:affiliation
Department of Blood and Marrow Transplantation, Section of Molecular Hematology and Therapy, The University of Texas M. D. Anderson Cancer Center, Houston, Texas 77030-4009, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, U.S. Gov't, Non-P.H.S., Research Support, Non-U.S. Gov't