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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
23
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pubmed:dateCreated |
2003-10-23
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pubmed:abstractText |
In Xenopus laevis, beta-catenin-mediated dorsal axis formation can be suppressed by overexpression of the HMG-box transcription factor XSOX3. Mutational analysis indicates that this effect is due not to the binding of XSOX3 to beta-catenin nor to its competition with beta-catenin-regulated TCF-type transcription factors for specific DNA binding sites, but rather to SOX3 binding to sites within the promoter of the early VegT- and beta-catenin-regulated dorsal-mesoderm-inducing gene Xnr5. Although B1-type SOX proteins, such as XSOX3, are commonly thought to act as transcriptional activators, XSOX3 acts as a transcriptional repressor of Xnr5 in both the intact embryo and animal caps injected with VegT RNA. Expression of a chimeric polypeptide composed of XSOX3 and a VP16 transcriptional activation domain or morpholino-induced decrease in endogenous XSOX3 polypeptide levels lead to an increase in Xnr5 expression, as does injection of an anti-XSOX3 antibody that inhibits XSOX3 DNA binding. These observations indicate that maternal XSOX3 acts in a novel manner to restrict Xnr5 expression to the vegetal hemisphere.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antibodies,
http://linkedlifedata.com/resource/pubmed/chemical/CTNNB1 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Cytoskeletal Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/DNA-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/High Mobility Group Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Homeodomain Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Nodal Signaling Ligands,
http://linkedlifedata.com/resource/pubmed/chemical/Nuclear Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Oligonucleotides, Antisense,
http://linkedlifedata.com/resource/pubmed/chemical/Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/SOX3 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/SOXB1 Transcription Factors,
http://linkedlifedata.com/resource/pubmed/chemical/Sox3 protein, Xenopus,
http://linkedlifedata.com/resource/pubmed/chemical/T-Box Domain Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Trans-Activators,
http://linkedlifedata.com/resource/pubmed/chemical/Transcription Factors,
http://linkedlifedata.com/resource/pubmed/chemical/VegT protein, Xenopus,
http://linkedlifedata.com/resource/pubmed/chemical/Xenopus Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Xnr5 protein, Xenopus,
http://linkedlifedata.com/resource/pubmed/chemical/beta Catenin,
http://linkedlifedata.com/resource/pubmed/chemical/beta-catenin protein, Xenopus,
http://linkedlifedata.com/resource/pubmed/chemical/siamois protein, Xenopus
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pubmed:status |
MEDLINE
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pubmed:month |
Dec
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pubmed:issn |
0950-1991
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
130
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
5609-24
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pubmed:dateRevised |
2011-11-17
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pubmed:meshHeading |
pubmed-meshheading:14522872-Amino Acid Sequence,
pubmed-meshheading:14522872-Animals,
pubmed-meshheading:14522872-Antibodies,
pubmed-meshheading:14522872-Base Sequence,
pubmed-meshheading:14522872-Body Patterning,
pubmed-meshheading:14522872-Cytoskeletal Proteins,
pubmed-meshheading:14522872-DNA-Binding Proteins,
pubmed-meshheading:14522872-Female,
pubmed-meshheading:14522872-Gene Expression Regulation, Developmental,
pubmed-meshheading:14522872-Genes, Reporter,
pubmed-meshheading:14522872-HeLa Cells,
pubmed-meshheading:14522872-High Mobility Group Proteins,
pubmed-meshheading:14522872-Homeodomain Proteins,
pubmed-meshheading:14522872-Humans,
pubmed-meshheading:14522872-Models, Molecular,
pubmed-meshheading:14522872-Molecular Sequence Data,
pubmed-meshheading:14522872-Nodal Signaling Ligands,
pubmed-meshheading:14522872-Nuclear Proteins,
pubmed-meshheading:14522872-Oligonucleotides, Antisense,
pubmed-meshheading:14522872-Oocytes,
pubmed-meshheading:14522872-Promoter Regions, Genetic,
pubmed-meshheading:14522872-Protein Structure, Tertiary,
pubmed-meshheading:14522872-Proteins,
pubmed-meshheading:14522872-SOXB1 Transcription Factors,
pubmed-meshheading:14522872-T-Box Domain Proteins,
pubmed-meshheading:14522872-Trans-Activators,
pubmed-meshheading:14522872-Transcription Factors,
pubmed-meshheading:14522872-Xenopus Proteins,
pubmed-meshheading:14522872-Xenopus laevis,
pubmed-meshheading:14522872-beta Catenin
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pubmed:year |
2003
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pubmed:articleTitle |
The beta-catenin/VegT-regulated early zygotic gene Xnr5 is a direct target of SOX3 regulation.
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pubmed:affiliation |
Molecular, Cellular and Developmental Biology, University of Colorado, Boulder, Colorado 80309-0347, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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