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rdf:type |
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lifeskim:mentions |
umls-concept:C0006556,
umls-concept:C0009015,
umls-concept:C0010798,
umls-concept:C0029047,
umls-concept:C0036108,
umls-concept:C0038302,
umls-concept:C0205065,
umls-concept:C0442043,
umls-concept:C0449774,
umls-concept:C0871161,
umls-concept:C1515670
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pubmed:issue |
1
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pubmed:dateCreated |
1992-12-31
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pubmed:databankReference |
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pubmed:abstractText |
A cDNA clone encoding cytochrome P-450c17 (17 alpha-hydroxylase/17,20-lyase) was isolated from a rainbow trout ovarian follicle cDNA library. The cDNA contained an open reading frame of 1,542 nucleotides encoding a protein of 514 amino acid residues. The amino acid sequence of trout P-450c17 shows a much greater homology with chicken P-450c17 than with that of human, bovine and rat. The trout P-450c17 expressed in non-steroidogenic mammalian COS-1 cells showed both 17 alpha-hydroxylase and 17,20-lyase activities. The cDNA only hybridized to a single species of mRNA (2.4 kb) isolated from rainbow trout ovaries; the 2.4 kb transcripts were abundant in trout ovaries during the later stages of oogenesis.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Apr
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pubmed:issn |
0014-5793
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
13
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pubmed:volume |
301
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
60-4
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:1451787-Aldehyde-Lyases,
pubmed-meshheading:1451787-Amino Acid Sequence,
pubmed-meshheading:1451787-Animals,
pubmed-meshheading:1451787-Base Sequence,
pubmed-meshheading:1451787-Cattle,
pubmed-meshheading:1451787-Cells, Cultured,
pubmed-meshheading:1451787-Cloning, Molecular,
pubmed-meshheading:1451787-Cytochrome P-450 Enzyme System,
pubmed-meshheading:1451787-Female,
pubmed-meshheading:1451787-Gene Library,
pubmed-meshheading:1451787-Humans,
pubmed-meshheading:1451787-Molecular Sequence Data,
pubmed-meshheading:1451787-Oogenesis,
pubmed-meshheading:1451787-Ovary,
pubmed-meshheading:1451787-Pregnenolone,
pubmed-meshheading:1451787-Progesterone,
pubmed-meshheading:1451787-RNA, Messenger,
pubmed-meshheading:1451787-Rats,
pubmed-meshheading:1451787-Recombinant Proteins,
pubmed-meshheading:1451787-Sequence Homology, Amino Acid,
pubmed-meshheading:1451787-Steroid 17-alpha-Hydroxylase,
pubmed-meshheading:1451787-Theca Cells,
pubmed-meshheading:1451787-Transfection,
pubmed-meshheading:1451787-Trout
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pubmed:year |
1992
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pubmed:articleTitle |
Rainbow trout cytochrome P-450c17 (17 alpha-hydroxylase/17,20-lyase). cDNA cloning, enzymatic properties and temporal pattern of ovarian P-450c17 mRNA expression during oogenesis.
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pubmed:affiliation |
Laboratory of Reproductive Biology, National Institute for Basic Biology, Okazaki, Japan.
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pubmed:publicationType |
Journal Article
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