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pubmed-article:1451598pubmed:abstractTextA rapid and reliable method for longitudinal studies on the degree of red cell chimerism following bone marrow transplantation of alpha-thalassemic recipient mice is presented. Blood obtained by tail clipping from transplanted mice was analyzed by measuring forward light scatter (FLS) distribution of red cells using a flow cytometer. Amplification and threshold of FLS were specifically adjusted. For flow cytometric analysis, the red cells needed to be suspended in hypotonic saline (103 mmol/l NaCl). Osmotic fragility testing showed that lysis of erythrocytes did not significantly influence the measurements. Flow cytometric measurement allowed for a rapid determination of the degree of red cell chimerism.lld:pubmed
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pubmed-article:1451598pubmed:dateRevised2006-11-15lld:pubmed
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pubmed-article:1451598pubmed:articleTitleFlow cytometric analysis of peripheral blood erythrocyte chimerism in alpha-thalassemic mice.lld:pubmed
pubmed-article:1451598pubmed:affiliationDepartment of Radiobiology, Erasmus University, Rotterdam, The Netherlands.lld:pubmed
pubmed-article:1451598pubmed:publicationTypeJournal Articlelld:pubmed
pubmed-article:1451598pubmed:publicationTypeResearch Support, Non-U.S. Gov'tlld:pubmed