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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
6
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pubmed:dateCreated |
1993-1-7
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pubmed:abstractText |
A rapid and reliable method for longitudinal studies on the degree of red cell chimerism following bone marrow transplantation of alpha-thalassemic recipient mice is presented. Blood obtained by tail clipping from transplanted mice was analyzed by measuring forward light scatter (FLS) distribution of red cells using a flow cytometer. Amplification and threshold of FLS were specifically adjusted. For flow cytometric analysis, the red cells needed to be suspended in hypotonic saline (103 mmol/l NaCl). Osmotic fragility testing showed that lysis of erythrocytes did not significantly influence the measurements. Flow cytometric measurement allowed for a rapid determination of the degree of red cell chimerism.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:status |
MEDLINE
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pubmed:issn |
0196-4763
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
13
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pubmed:geneSymbol |
Hba<up>th</up>
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
659-62
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:1451598-Animals,
pubmed-meshheading:1451598-Bone Marrow Transplantation,
pubmed-meshheading:1451598-Cell Size,
pubmed-meshheading:1451598-Erythrocytes,
pubmed-meshheading:1451598-Erythrocytes, Abnormal,
pubmed-meshheading:1451598-Erythropoiesis,
pubmed-meshheading:1451598-Flow Cytometry,
pubmed-meshheading:1451598-Graft Survival,
pubmed-meshheading:1451598-Longitudinal Studies,
pubmed-meshheading:1451598-Mice,
pubmed-meshheading:1451598-Mice, Inbred BALB C,
pubmed-meshheading:1451598-Nephelometry and Turbidimetry,
pubmed-meshheading:1451598-Osmotic Fragility,
pubmed-meshheading:1451598-Postoperative Period,
pubmed-meshheading:1451598-Radiation Chimera,
pubmed-meshheading:1451598-Transplantation, Homologous,
pubmed-meshheading:1451598-Whole-Body Irradiation,
pubmed-meshheading:1451598-alpha-Thalassemia
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pubmed:year |
1992
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pubmed:articleTitle |
Flow cytometric analysis of peripheral blood erythrocyte chimerism in alpha-thalassemic mice.
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pubmed:affiliation |
Department of Radiobiology, Erasmus University, Rotterdam, The Netherlands.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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