Linked Life Data

1451333

Source:http://linkedlifedata.com/resource/pubmed/id/1451333

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1993-1-7
pubmed:abstractText
We have previously demonstrated that IFN-alpha/beta, poly(I:C) (an inducer of IFN-alpha/beta), or IFN-gamma can inhibit the ability of KLH-pulsed peritoneal macrophages (M phi) to induce the proliferation of syngeneic, KLH-immune T lymphocytes from CBA/J mice. In this study we investigated whether the mechanism by which poly(I:C) inhibits M phi-induced, antigen-specific T cell proliferation involved decreased cytokine production by poly(I:C) treated KLH-pulsed M phi or by T cells cultured with these M phi. The production of IL-2 by T cells cultured with poly(I:C)-treated, KLH-pulsed M phi was decreased by 80%; however, addition of exogenous rIL-2 could not restore proliferation. Although IL-1 production by poly(I:C)-treated M phi was comparable to the level produced by saline-treated, KLH-pulsed M phi controls, addition of exogenous rIL-1 was still examined to explore the possibility that a greater amount of IL-1 may be needed to induce T cell proliferation with poly(I:C)-treated, KLH-pulsed M phi. Increasing concentrations of rIL-1 alone or with rIL-6 did not abrogate the inhibition of M phi-induced, antigen-specific T cell proliferation by poly(I:C). Interestingly, the addition of combinations of IL-1 and IL-6 increased the proliferation of T cells in response to KLH presented by either saline- or poly(I:C)-treated M phi. The effect of the combination of rIL-1 and rIL-6 was synergistic in that addition of either monokine alone had no effect on T cell proliferation. These results suggest that although poly(I:C)-induced inhibition of T cell proliferation is not due to insufficient quantities of IL-1, IL-2, or IL-6, a combination of IL-1 and IL-6 can augment proliferation of freshly isolated T cells in response to antigen presented by freshly isolated accessory cells.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0090-1229
pubmed:author
pubmed:issnType
Print
pubmed:volume
65
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
300-7
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1992
pubmed:articleTitle
Effect of exogenous cytokines on the inhibition of macrophage-induced, antigen-specific T cell proliferation by poly(I:C).
pubmed:affiliation
Department of Microbiology and Immunology, Medical College of Pennsylvania, Philadelphia 19129.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.