Source:http://linkedlifedata.com/resource/pubmed/id/14513314
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
1-2
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| pubmed:dateCreated |
2003-9-26
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| pubmed:abstractText |
The localization of the actin-monomer-binding protein profilin during the cell cycle of living Tradescantia virginiana stamen hair cells has been studied by microinjection of a fluorescently labeled analog of the protein. In contrast to previously published studies performed on chemically fixed animal cells, we do not find a specific colocalization of profilin with actin filament arrays. Our results show that, besides a general cytoplasmic distribution, profilin specifically accumulates in the nucleus in interphase and prophase cells. This nuclear localization was confirmed by means of electron microscopic immunolocalization of endogenous profilin (in Gibasis scheldiana stamen hair cells). During mitosis, as the nuclear envelope and nuclear matrix break down at the onset of prometaphase, the nuclear profilin redistributes equally into the accessible volume (cytosol) of the cell. During metaphase and anaphase no specific localization of profilin can be observed associated with the mitotic apparatus. However, during telophase, as nuclear envelopes and nuclear matrices re-form and the sister chromatids start to decondense, a subset of the microinjected profilin again localizes to the nucleus. No accumulation of profilin could be observed in the phragmoplast, where a distinct array of actin filaments exists. The function of profilin in the nucleus remains unclear.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Actins,
http://linkedlifedata.com/resource/pubmed/chemical/Contractile Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Microfilament Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Phosphatidylinositol 4,5-Diphosphate,
http://linkedlifedata.com/resource/pubmed/chemical/Plant Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Profilins
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| pubmed:status |
MEDLINE
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| pubmed:month |
Sep
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| pubmed:issn |
0033-183X
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| pubmed:author | |
| pubmed:issnType |
Print
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| pubmed:volume |
222
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
85-95
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| pubmed:dateRevised |
2007-11-15
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| pubmed:meshHeading |
pubmed-meshheading:14513314-Actins,
pubmed-meshheading:14513314-Animals,
pubmed-meshheading:14513314-Cell Cycle,
pubmed-meshheading:14513314-Cell Nucleus,
pubmed-meshheading:14513314-Cells, Cultured,
pubmed-meshheading:14513314-Chickens,
pubmed-meshheading:14513314-Contractile Proteins,
pubmed-meshheading:14513314-Fluorescent Antibody Technique, Indirect,
pubmed-meshheading:14513314-Hair Cells, Auditory,
pubmed-meshheading:14513314-Male,
pubmed-meshheading:14513314-Microfilament Proteins,
pubmed-meshheading:14513314-Microinjections,
pubmed-meshheading:14513314-Microscopy, Confocal,
pubmed-meshheading:14513314-Phosphatidylinositol 4,5-Diphosphate,
pubmed-meshheading:14513314-Plant Proteins,
pubmed-meshheading:14513314-Pollen,
pubmed-meshheading:14513314-Profilins,
pubmed-meshheading:14513314-Tradescantia
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| pubmed:year |
2003
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| pubmed:articleTitle |
Nuclear localization of profilin during the cell cycle in Tradescantia virginiana stamen hair cells.
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| pubmed:affiliation |
North Shore University Hospital, Manhasset, New York, USA.
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| pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, Non-P.H.S.,
Research Support, Non-U.S. Gov't
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