Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
2003-9-26
pubmed:abstractText
Genes that determine the invasive capacity of the invasive front of malignant melanomas (MM) have not yet been systematically investigated in vivo. Therefore, we combined laser pressure catapulting (LPC) microdissection with cDNA microarray technology (DermArray, Research Genetics, representing about 5700 genes) to systematically analyse differences in gene expression profiles between the invasive margin and the tumour centre in nine cases of vertical growth phase MM. Signal-to-noise statistical algorithms combined with hierarchical clustering were performed to determine class-separating genes. The gene encoding phosphoenolpyruvate carboxykinase 1 (PEPCK), the Homo sapiens gene similar to Saccharomyces cerevisiae SSM4 (TEB4), the gene encoding ribosomal protein L19, the Homo sapiens gene similar to the Aspergillus nidulans SudD (a suppressor of the bimD6 homologue), the gene encoding the interleukin-3 receptor alpha subunit, the gene encoding the inositol 1,4,5-triphosphate 3-kinase isoenzyme, and three anonymous expressed sequence tags were identified as class-separating genes. These genes significantly discriminate between the invasive front and the tumour centre. Using this set of genes, 15 out of 18 LPC-dissected MM regions could be grouped correctly. We conclude that the candidate genes identified could spark further research on MM progression and may provide novel prognostic parameters.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
http://linkedlifedata.com/resource/pubmed/chemical/DNA, Complementary, http://linkedlifedata.com/resource/pubmed/chemical/Fungal Proteins, http://linkedlifedata.com/resource/pubmed/chemical/IL3RA protein, human, http://linkedlifedata.com/resource/pubmed/chemical/Inositol 1,4,5-trisphosphate..., http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-3 Receptor alpha Subunit, http://linkedlifedata.com/resource/pubmed/chemical/Phosphotransferases (Alcohol Group..., http://linkedlifedata.com/resource/pubmed/chemical/Protein Isoforms, http://linkedlifedata.com/resource/pubmed/chemical/Protein-Serine-Threonine Kinases, http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Interleukin-3, http://linkedlifedata.com/resource/pubmed/chemical/SUDD protein, Emericella nidulans, http://linkedlifedata.com/resource/pubmed/chemical/phosphoenolpyruvate carboxylase...
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0960-8931
pubmed:author
pubmed:issnType
Print
pubmed:volume
13
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
503-9
pubmed:dateRevised
2007-11-15
pubmed:meshHeading
pubmed-meshheading:14512792-Algorithms, pubmed-meshheading:14512792-Cluster Analysis, pubmed-meshheading:14512792-DNA, Complementary, pubmed-meshheading:14512792-Disease Progression, pubmed-meshheading:14512792-Fungal Proteins, pubmed-meshheading:14512792-Gene Expression Regulation, Neoplastic, pubmed-meshheading:14512792-Humans, pubmed-meshheading:14512792-Interleukin-3 Receptor alpha Subunit, pubmed-meshheading:14512792-Lasers, pubmed-meshheading:14512792-Melanoma, pubmed-meshheading:14512792-Neoplasm Invasiveness, pubmed-meshheading:14512792-Nucleic Acid Hybridization, pubmed-meshheading:14512792-Oligonucleotide Array Sequence Analysis, pubmed-meshheading:14512792-Phosphotransferases (Alcohol Group Acceptor), pubmed-meshheading:14512792-Prognosis, pubmed-meshheading:14512792-Protein Isoforms, pubmed-meshheading:14512792-Protein-Serine-Threonine Kinases, pubmed-meshheading:14512792-Receptors, Interleukin-3, pubmed-meshheading:14512792-Up-Regulation
pubmed:year
2003
pubmed:articleTitle
Discrimination between gene expression patterns in the invasive margin and the tumour core of malignant melanomas.
pubmed:affiliation
Department of Dermatology, University of Regensburg, Franz-Josef-Strauss-Allee 11, D-93053 Regensburg, Germany. alexander.roesch@klinik.uni-regensburg.de
pubmed:publicationType
Journal Article