Source:http://linkedlifedata.com/resource/pubmed/id/14512159
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
1-2
|
| pubmed:dateCreated |
2003-9-26
|
| pubmed:abstractText |
Chemokines have a pivotal role in the selective mediation and amplification of inflammation. The CNS vascular endothelial cells, which form part of the blood-brain barrier (BBB) and blood-retinal barrier (BRB), are ideally situated to present chemokines to circulating lymphocytes leading to their recruitment. Monocyte-chemoattractant protein-1 (MCP-1), also known as CCL2, a potent chemoattractant of T cells and monocytes, has been implicated in inflammatory and angio-proliferative brain and retinal disease. In this study, MCP-1 expression by CNS endothelial cells was investigated in vitro. Rat brain (GP8/3.9) and retinal (JG2/1) vascular endothelial cell lines expressed MCP-1 constitutively in vitro as assessed by immunocytochemistry and enzyme linked immunosorbant assay (ELISA). Upregulation of secreted MCP-1 was observed following activation with the pro-inflammatory cytokines TNF-alpha, IL-1 beta and IFN-gamma, and was reduced following dexamethasone treatment. Functional chemotactic activity of brain and retinal endothelial cell supernatants was demonstrated in an in vitro chemotaxis assay, which was inhibited by anti-MCP-1 antibodies. These findings suggest that endothelial cell-derived MCP-1 plays a key role in leukocyte recruitment across the blood-brain and blood-retinal barriers in vivo.
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| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Chemokine CCL2,
http://linkedlifedata.com/resource/pubmed/chemical/Cytokines,
http://linkedlifedata.com/resource/pubmed/chemical/Dexamethasone,
http://linkedlifedata.com/resource/pubmed/chemical/Immunosuppressive Agents,
http://linkedlifedata.com/resource/pubmed/chemical/Interferon-gamma,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-1,
http://linkedlifedata.com/resource/pubmed/chemical/Tumor Necrosis Factor-alpha
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Sep
|
| pubmed:issn |
0165-5728
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| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
142
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
1-9
|
| pubmed:dateRevised |
2008-11-21
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| pubmed:meshHeading |
pubmed-meshheading:14512159-Animals,
pubmed-meshheading:14512159-Brain,
pubmed-meshheading:14512159-Cell Line,
pubmed-meshheading:14512159-Cell-Free System,
pubmed-meshheading:14512159-Chemokine CCL2,
pubmed-meshheading:14512159-Chemotaxis, Leukocyte,
pubmed-meshheading:14512159-Cytokines,
pubmed-meshheading:14512159-Dexamethasone,
pubmed-meshheading:14512159-Endothelium, Vascular,
pubmed-meshheading:14512159-Female,
pubmed-meshheading:14512159-Immunosuppressive Agents,
pubmed-meshheading:14512159-Interferon-gamma,
pubmed-meshheading:14512159-Interleukin-1,
pubmed-meshheading:14512159-Intracellular Fluid,
pubmed-meshheading:14512159-Microcirculation,
pubmed-meshheading:14512159-Rats,
pubmed-meshheading:14512159-Rats, Inbred Lew,
pubmed-meshheading:14512159-Retinal Vessels,
pubmed-meshheading:14512159-Tumor Necrosis Factor-alpha
|
| pubmed:year |
2003
|
| pubmed:articleTitle |
Cytokine regulation of MCP-1 expression in brain and retinal microvascular endothelial cells.
|
| pubmed:affiliation |
Department of Neurology, Addenbrooke's NHS Trust, Cambridge CB2 2QQ, UK.
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| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|