Linked Life Data

14510490

Source:http://linkedlifedata.com/resource/pubmed/id/14510490

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
2003-9-26
pubmed:abstractText
Bacterial ribonuclease P (RNase P) contains a catalytic RNA that cleaves precursor tRNA to form the 5'-end of mature tRNA. Bacterial RNase P mainly recognizes the acceptor stem and T arm modules of tRNA molecules. The region consisting of T arm, acceptor stem and 3' CCA motif in the tRNA is generally termed "top half", and the region consisting of the others, anticodon arm, extra loop and D arm, is called "bottom half". The stems in the top half contribute to recognition, but effects of the bottom half have not been elucidated. To study the effects of the bottom half on the RNase P recognition, we have synthesized several mutant substrates that have the bottom half on different positions along the top half stem. Most of these mutants were cleaved by Escherichia coli RNase P precisely at the expected position, but the cleavage efficiencies were very different especially at low Mg2+ concentration. We also found that RNase P holoenzyme prefered somewhat mutated tRNA precursor to the wild-type tRNA precursor.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:author
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
281-2
pubmed:dateRevised
2006-11-30
pubmed:meshHeading
pubmed:year
2003
pubmed:articleTitle
Recognition of tRNA bottom half by bacterial ribonuclease P.
pubmed:affiliation
Division of Bioscience and Biotechnology, Department of Ecological Engineering, Toyohashi University of Technology, Hibarigaoka 1-1, Tempaku-cho, Toyohashi, Aichi 441-8580, Japan.
pubmed:publicationType
Journal Article