Source:http://linkedlifedata.com/resource/pubmed/id/14510433
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
3
|
| pubmed:dateCreated |
2003-9-26
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| pubmed:abstractText |
Useful technique to clip designated short RNA fragments from long substrates has been prepared by combining oligonucleotides bearing two acridine groups and lanthanide(III) ions. The substrate RNA is site-selectively activated at two designated phosphodiester linkages by complementary bis-acridine-modified DNA, and is promptly cleaved by lanthanide(III) ions to produce short RNA fragment between the two scission sites. By applying this technique, efficient genotyping method for single nucleotide polymorphism (SNP) have been developed.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:author | |
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
167-8
|
| pubmed:dateRevised |
2006-11-30
|
| pubmed:meshHeading |
pubmed-meshheading:14510433-Acridines,
pubmed-meshheading:14510433-Base Sequence,
pubmed-meshheading:14510433-Chromatography, High Pressure Liquid,
pubmed-meshheading:14510433-DNA,
pubmed-meshheading:14510433-Electrophoresis, Polyacrylamide Gel,
pubmed-meshheading:14510433-RNA,
pubmed-meshheading:14510433-Spectrometry, Mass, Matrix-Assisted Laser...
|
| pubmed:year |
2003
|
| pubmed:articleTitle |
Tandem site-selective RNA scission utilizing acridine-DNA conjugates.
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| pubmed:affiliation |
Research Center for Advanced Science and Technology, The University of Tokyo, Komaba, Meguro-ku, Tokyo 153-8904, Japan.
|
| pubmed:publicationType |
Journal Article
|