Linked Life Data

14510386

Source:http://linkedlifedata.com/resource/pubmed/id/14510386

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
2003-9-26
pubmed:abstractText
Pyrene-labeled 2'-O-methyloligoribonucleotide (OMUpy) and 5'-Ru(II) complex labeled oligodeoxyribonucleotie (Ru-probe) were prepared as the fluorescence probes to detect acceptor sites of antisense molecules on native folded RNA under the physiological condition. OMUpy showed the remarkable increase of the fluorescence intensity (334-fold at 375 nm) only when hybridized with complementary oligoRNA. When OMUpy was applied to E. coli 16S-rRNA, the fluorescence intensities were increased in a sequence specific manner. The rotational correlation time of Ru-probe complementary to 16S-rRNA were largely dependent on the sequence of Ru-probe. These results suggest that the accessibility of the antisense molecules for RNA can be evaluated by those fluorescent probes.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:author
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
73-4
pubmed:dateRevised
2006-11-30
pubmed:meshHeading
pubmed:year
2003
pubmed:articleTitle
Detection of acceptor sites for antisense oligonucleotides on native folded RNA by fluorescence-labeled oligonucleotide.
pubmed:affiliation
Department of Polymer Science and Engineering, Kyoto Institute of Technology, Sakyo-ku, Kyoto 606-8585, Japan.
pubmed:publicationType
Journal Article