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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
6
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pubmed:dateCreated |
2003-9-24
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pubmed:abstractText |
HIV release requires TSG101, a cellular factor that sorts proteins into vesicles that bud into multivesicular bodies (MVB). To test whether other proteins involved in MVB biogenesis (the class E proteins) also participate in HIV release, we identified 22 candidate human class E proteins. These proteins were connected into a coherent network by 43 different protein-protein interactions, with AIP1 playing a key role in linking complexes that act early (TSG101/ESCRT-I) and late (CHMP4/ESCRT-III) in the pathway. AIP1 also binds the HIV-1 p6(Gag) and EIAV p9(Gag) proteins, indicating that it can function directly in virus budding. Human class E proteins were found in HIV-1 particles, and dominant-negative mutants of late-acting human class E proteins arrested HIV-1 budding through plasmal and endosomal membranes. These studies define a protein network required for human MVB biogenesis and indicate that the entire network participates in the release of HIV and probably many other viruses.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/DNA-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Endosomal Sorting Complexes...,
http://linkedlifedata.com/resource/pubmed/chemical/Gene Products, gag,
http://linkedlifedata.com/resource/pubmed/chemical/Microfilament Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Transcription Factors,
http://linkedlifedata.com/resource/pubmed/chemical/Tsg101 protein,
http://linkedlifedata.com/resource/pubmed/chemical/Viral Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/actin interacting protein 1,
http://linkedlifedata.com/resource/pubmed/chemical/gag Gene Products, Human...,
http://linkedlifedata.com/resource/pubmed/chemical/p6 gag protein, Human...
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pubmed:status |
MEDLINE
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pubmed:month |
Sep
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pubmed:issn |
0092-8674
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pubmed:author |
pubmed-author:ChungHyo-YoungHY,
pubmed-author:CimboraDaniel MDM,
pubmed-author:DavisThaylonT,
pubmed-author:HeGong-PingGP,
pubmed-author:KaplanJerryJ,
pubmed-author:KräusslichHans-GeorgHG,
pubmed-author:MüllerBarbaraB,
pubmed-author:MorhamScott GSG,
pubmed-author:MoritaEijiE,
pubmed-author:ScottAnnaA,
pubmed-author:StuchellMelissaM,
pubmed-author:SundquistWesley IWI,
pubmed-author:WangHubert EHE,
pubmed-author:WardDiane MDM,
pubmed-author:von SchwedlerUta KUK
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pubmed:issnType |
Print
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pubmed:day |
19
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pubmed:volume |
114
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
701-13
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:14505570-Animals,
pubmed-meshheading:14505570-COS Cells,
pubmed-meshheading:14505570-Cell Compartmentation,
pubmed-meshheading:14505570-Cell Line,
pubmed-meshheading:14505570-Cell Membrane,
pubmed-meshheading:14505570-DNA-Binding Proteins,
pubmed-meshheading:14505570-Endosomal Sorting Complexes Required for Transport,
pubmed-meshheading:14505570-Endosomes,
pubmed-meshheading:14505570-Gene Products, gag,
pubmed-meshheading:14505570-HIV-1,
pubmed-meshheading:14505570-Humans,
pubmed-meshheading:14505570-Microfilament Proteins,
pubmed-meshheading:14505570-Microscopy, Electron,
pubmed-meshheading:14505570-Models, Biological,
pubmed-meshheading:14505570-Mutation,
pubmed-meshheading:14505570-Protein Binding,
pubmed-meshheading:14505570-Proteins,
pubmed-meshheading:14505570-Transcription Factors,
pubmed-meshheading:14505570-Transport Vesicles,
pubmed-meshheading:14505570-Viral Proteins,
pubmed-meshheading:14505570-Virus Shedding,
pubmed-meshheading:14505570-gag Gene Products, Human Immunodeficiency Virus
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pubmed:year |
2003
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pubmed:articleTitle |
The protein network of HIV budding.
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pubmed:affiliation |
Department of Biochemistry, University of Utah, Salt Lake City, UT 84132, USA.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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