Source:http://linkedlifedata.com/resource/pubmed/id/14504034
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
3-4
|
| pubmed:dateCreated |
2003-9-23
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| pubmed:abstractText |
Western blots analyses and gelatin zymography established the presence of matrix metalloproteinase (MMP)-2 and -9 in the forming zone of rat incisor. Light microscope immunohistochemistry carried out on undemineralized material provided evidence for strong MMP-2 staining in the secretory ameloblasts, odontoblasts, in the enamel organ, and in the pulp. A weaker staining was observed in predentin and in the outer part of the forming enamel. Using MMP-9 antibodies, the staining was generally weak, except for the secretory ameloblasts that were positively stained. Electron microscopic immunohistochemistry of undemineralized sections revealed a close association between gold-antibodies complexes and cytoskeletal microfilaments in the cytosol of secretory ameloblasts and odontoblasts, within the rough endoplasmic reticulum and along the plasma membrane. The striking feature of MMP-2 and -9 electron immunostaining was the particularly high labeling in the mantle dentin. By contrast, staining of tissue inhibitors of metalloproteinases (TIMP-1 and -2) was lowest in this region. We suggest that this uneven distribution may have some functional implications.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Matrix Metalloproteinase 2,
http://linkedlifedata.com/resource/pubmed/chemical/Matrix Metalloproteinase 9,
http://linkedlifedata.com/resource/pubmed/chemical/Tissue Inhibitor of...,
http://linkedlifedata.com/resource/pubmed/chemical/Tissue Inhibitor of...
|
| pubmed:status |
MEDLINE
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| pubmed:issn |
0300-8207
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| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
44
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| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
143-53
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| pubmed:dateRevised |
2011-11-17
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| pubmed:meshHeading |
pubmed-meshheading:14504034-Actin Cytoskeleton,
pubmed-meshheading:14504034-Ameloblasts,
pubmed-meshheading:14504034-Animals,
pubmed-meshheading:14504034-Cell Membrane,
pubmed-meshheading:14504034-Dental Pulp Cavity,
pubmed-meshheading:14504034-Dentin,
pubmed-meshheading:14504034-Enamel Organ,
pubmed-meshheading:14504034-Endoplasmic Reticulum, Rough,
pubmed-meshheading:14504034-Immunohistochemistry,
pubmed-meshheading:14504034-Incisor,
pubmed-meshheading:14504034-Male,
pubmed-meshheading:14504034-Matrix Metalloproteinase 2,
pubmed-meshheading:14504034-Matrix Metalloproteinase 9,
pubmed-meshheading:14504034-Microscopy, Electron,
pubmed-meshheading:14504034-Odontoblasts,
pubmed-meshheading:14504034-Rats,
pubmed-meshheading:14504034-Rats, Sprague-Dawley,
pubmed-meshheading:14504034-Tissue Inhibitor of Metalloproteinase-1,
pubmed-meshheading:14504034-Tissue Inhibitor of Metalloproteinase-2
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| pubmed:year |
2003
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| pubmed:articleTitle |
Immunohistochemical localization of MMP-2, MMP-9, TIMP-1, and TIMP-2 in the forming rat incisor.
|
| pubmed:affiliation |
Groupe Matrices Extracellulaires et Biominéralisations, Faculté de Chirurgie Dentaire, Université Paris V, Montrouge, France. mgoldod@aol.com
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| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|