Source:http://linkedlifedata.com/resource/pubmed/id/14503641
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
8
|
| pubmed:dateCreated |
2003-9-23
|
| pubmed:abstractText |
Cerebrospinal fluid (CSF) has been rediscovered in the post-genomic era as a great source of potential protein biomarkers for various diseases. The source allows rapid screening, low sample consumption, and accurate protein identification by proteomic technology. In the present study, we identified 2 candidate tumor-related proteins, N-myc oncoprotein and low-molecular weight caldesmon (l-CaD), in CSF samples of patients with primary brain tumors by using 2-dimensional polyacrylamide gel electrophoresis (2D PAGE), followed by matrix-assisted laser desorption/ionization-time of flight-mass spectrometry (MALDI-TOF-MS) analysis. N-myc and l-CaD were related to tumor cell nuclei and blood vessels, respectively, in tissue sections of the tumor biopsies taken from the same patients from whom CSF was processed. N-myc oncoprotein and l-CaD have not been detected in CSF before. The practical value of these proteins as possible tumor markers, prognosticators, or their utility in monitoring response to chemotherapy is currently a subject of investigation. It is concluded that the combination of 2D PAGE and MALDI-TOF-MS is successful as an unbiased global screening tool for CSF.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Aug
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| pubmed:issn |
0022-3069
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| pubmed:author | |
| pubmed:issnType |
Print
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| pubmed:volume |
62
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
855-62
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| pubmed:dateRevised |
2006-11-15
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| pubmed:meshHeading |
pubmed-meshheading:14503641-Adolescent,
pubmed-meshheading:14503641-Adult,
pubmed-meshheading:14503641-Brain Neoplasms,
pubmed-meshheading:14503641-Calmodulin-Binding Proteins,
pubmed-meshheading:14503641-Child,
pubmed-meshheading:14503641-Child, Preschool,
pubmed-meshheading:14503641-Databases, Protein,
pubmed-meshheading:14503641-Electrophoresis, Gel, Two-Dimensional,
pubmed-meshheading:14503641-Female,
pubmed-meshheading:14503641-Glioma,
pubmed-meshheading:14503641-Humans,
pubmed-meshheading:14503641-Immunohistochemistry,
pubmed-meshheading:14503641-Male,
pubmed-meshheading:14503641-Middle Aged,
pubmed-meshheading:14503641-Neoplasm Proteins,
pubmed-meshheading:14503641-Proteomics,
pubmed-meshheading:14503641-Proto-Oncogene Proteins c-myc,
pubmed-meshheading:14503641-Spectrometry, Mass, Matrix-Assisted Laser...,
pubmed-meshheading:14503641-Staining and Labeling
|
| pubmed:year |
2003
|
| pubmed:articleTitle |
Identification of tumor-related proteins by proteomic analysis of cerebrospinal fluid from patients with primary brain tumors.
|
| pubmed:affiliation |
Department of Pathology, Erasmus Medical Center Rotterdam, Rotterdam, The Netherlands.
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| pubmed:publicationType |
Journal Article,
Comparative Study
|