| pubmed-article:14501049 | pubmed:abstractText | RNA suppression approaches provide a rapid survey of gene function. Of these approaches (i.e., antisense oligonucleotides, ribozymes, and RNA interference), ribozymes offer significant advantages by operating as stringent site-specific ribonucleases. The purpose of this chapter is to provide some guidelines and protocols for the use of ribozymes to identify the functions of closely related members of the G protein gamma subunit family. Improvements in their design and delivery will be discussed, including the use of "second generation" ribozymes targeted against multiple cleavage sites. | lld:pubmed |
