Source:http://linkedlifedata.com/resource/pubmed/id/14500660
Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
7
|
| pubmed:dateCreated |
2003-9-22
|
| pubmed:abstractText |
IL-4, primarily produced by T cells, mast cells, and basophiles, is a cytokine which has pleiotropic effects on the immune system. IL-4 induces T cells to differentiate to Th2 cells and activated B lymphocytes to proliferate and to synthesize IgE and IgG1. IL-4 is particularly important for the development and perpetuation of asthma and allergy. Stat6 is the protein activated by signal transduction through the IL-4R, and studies with knockout mice demonstrate that Stat6 is critical for a number of IL-4-mediated functions including Th2 development and production of IgE. In the present study, novel IL-4- and Stat6-regulated genes were discovered by using Stat6(-/-) mice and Affymetrix oligonucleotide arrays. Genes regulated by IL-4 were identified by comparing the gene expression profile of the wild-type T cells induced to polarize to the Th2 direction (CD3/CD28 activation + IL-4) to gene expression profile of the cells induced to proliferate (CD3/CD28 activation alone). Stat6-regulated genes were identified by comparing the cells isolated from the wild-type and Stat6(-/-) mice; in this experiment the cells were induced to differentiate to the Th2 direction (CD3/CD28 activation + IL-4). Our study demonstrates that a number a novel genes are regulated by IL-4 through Stat6-dependent and -independent pathways. Moreover, elucidation of kinetics of gene expression at early stages of cell differentiation reveals several genes regulated rapidly during the process, suggesting their importance for the differentiation process.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
AIM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Immediate-Early Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-4,
http://linkedlifedata.com/resource/pubmed/chemical/STAT6 Transcription Factor,
http://linkedlifedata.com/resource/pubmed/chemical/Stat6 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Taq Polymerase,
http://linkedlifedata.com/resource/pubmed/chemical/Trans-Activators
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Oct
|
| pubmed:issn |
0022-1767
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
1
|
| pubmed:volume |
171
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
3627-35
|
| pubmed:dateRevised |
2006-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:14500660-Animals,
pubmed-meshheading:14500660-CD4-Positive T-Lymphocytes,
pubmed-meshheading:14500660-Cell Differentiation,
pubmed-meshheading:14500660-Cells, Cultured,
pubmed-meshheading:14500660-Flow Cytometry,
pubmed-meshheading:14500660-Gene Expression Profiling,
pubmed-meshheading:14500660-Gene Expression Regulation,
pubmed-meshheading:14500660-Immediate-Early Proteins,
pubmed-meshheading:14500660-Interleukin-4,
pubmed-meshheading:14500660-Kinetics,
pubmed-meshheading:14500660-Mice,
pubmed-meshheading:14500660-Mice, Inbred BALB C,
pubmed-meshheading:14500660-Mice, Knockout,
pubmed-meshheading:14500660-Oligonucleotide Array Sequence Analysis,
pubmed-meshheading:14500660-Polymerase Chain Reaction,
pubmed-meshheading:14500660-STAT6 Transcription Factor,
pubmed-meshheading:14500660-T-Lymphocyte Subsets,
pubmed-meshheading:14500660-Taq Polymerase,
pubmed-meshheading:14500660-Th2 Cells,
pubmed-meshheading:14500660-Trans-Activators
|
| pubmed:year |
2003
|
| pubmed:articleTitle |
Identification of novel IL-4/Stat6-regulated genes in T lymphocytes.
|
| pubmed:affiliation |
Turku Centre for Biotechnology, Drug Discovery Graduate School, University of Turku, Turku, Finland. zhi.chen@btk.utu.fi
|
| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|