Linked Life Data

1449605

Source:http://linkedlifedata.com/resource/pubmed/id/1449605

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
7
pubmed:dateCreated
1993-1-7
pubmed:abstractText
A cDNA clone, p14-6, which has an anti-oncogene activity on the v-Ki-Ras oncogene-transformed malignant cell line DT, was found. This clone was recovered from the revertant R14 cells, which had been isolated by transfections of DT cells with a normal human fibroblast cDNA library cloned in pcD2, an Okayama-Berg vector. When transfected into DT cells, p14-6 clone gave rise to phenotypical flat reversion in 5-15% of DT transfectant colonies. The p14-6-transfected flat cell line, RR, was proven to be a true revertant with significantly reduced malignancy by in vitro and in vivo malignancy tests. All other clones recovered from R14 cells were unable to cause this reversion. Molecular hybridizations showed that the p14-6 was inserted into RR genome as tandem repeats, and no structural change was found in the v-Ki-Ras oncogene in RR genome. These facts suggest that the anti-oncogene activity of the p14-6 clone on the DT cells may be exerted through expression of the cDNA contained in this clone.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
1001-652X
pubmed:author
pubmed:issnType
Print
pubmed:volume
35
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
822-33
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1992
pubmed:articleTitle
A cDNA clone with anti-oncogene activity.
pubmed:affiliation
Shanghai Institute of Biochemistry, Academia Sinica, PRC.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't