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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
1992-12-30
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pubmed:abstractText |
In order to locate the receptor-binding region of human interleukin-6 (IL-6), twelve peptide fragments were prepared by digestion of IL-6 with lysylendopeptidase. A significant activity of the receptor-binding was observed only for a peptide Ile88-Lys121, although the activity was estimated at 10(4)-fold less than that of intact IL-6. Solution structure of the peptide Ile88-Lys121 was analyzed by using two-dimensional nuclear magnetic resonance (NMR) spectroscopy. The results indicate the presence of alpha-helices in the regions Leu93-Phe106 and Glu110-Ser119. On the basis of the NMR data, we also prepared two peptides. Four-fold less binding activity than that of the peptide Ile88-Lys121 was observed for the peptide Ile88-Arg105, but no activity for the peptide Glu110-Lys121. These results suggest that the helical peptide Ile88-Arg105 composes a part of the receptor-binding region.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Nov
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pubmed:issn |
0006-291X
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
30
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pubmed:volume |
189
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
211-20
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:1449477-Amino Acid Sequence,
pubmed-meshheading:1449477-Binding Sites,
pubmed-meshheading:1449477-Chromatography, High Pressure Liquid,
pubmed-meshheading:1449477-Circular Dichroism,
pubmed-meshheading:1449477-Humans,
pubmed-meshheading:1449477-Interleukin-6,
pubmed-meshheading:1449477-Kinetics,
pubmed-meshheading:1449477-Magnetic Resonance Spectroscopy,
pubmed-meshheading:1449477-Models, Structural,
pubmed-meshheading:1449477-Molecular Sequence Data,
pubmed-meshheading:1449477-Peptide Fragments,
pubmed-meshheading:1449477-Peptides,
pubmed-meshheading:1449477-Protein Conformation,
pubmed-meshheading:1449477-Protein Folding,
pubmed-meshheading:1449477-Receptors, Immunologic,
pubmed-meshheading:1449477-Receptors, Interleukin-6,
pubmed-meshheading:1449477-Serine Endopeptidases
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pubmed:year |
1992
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pubmed:articleTitle |
A receptor-binding peptide from human interleukin-6: isolation and a proton nuclear magnetic resonance study.
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pubmed:affiliation |
Faculty of Pharmaceutical Sciences, University of Tokyo, Japan.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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