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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
1992-12-30
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pubmed:abstractText |
The methanolysis of small amounts of purified phosphoglycerides and sphingomyelin was studied and a quantitative comparison of five methods for the methanolysis of standard phosphoglycerides was made. These methods were based on methanolysis with boron trifluoride-methanol, methanolic sodium methoxide (at ambient temperature and with heating) and methanolic sulphuric acid. A further method was based on saponification with methanolic sodium hydroxide and subsequent esterification with boron trifluoride-methanol. Under the experimental conditions, only the sodium methoxide-catalysed method at ambient temperature gave complete methanolysis of phosphoglycerides. For methanolysis of sphingomyelin, boron trifluoride-methanol, methanolic sulphuric acid and methanolic hydrochloric acid were used. It was found that complete methanolysis of sphingomyelin takes 15 h at 90 degrees C. Based on these results, procedures for the methanolysis of phosphoglycerides and sphingomyelin separated by high-performance liquid chromatography are presented.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
0021-9673
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
21
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pubmed:volume |
607
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
55-67
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pubmed:dateRevised |
2003-11-14
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pubmed:meshHeading |
pubmed-meshheading:1447360-Animals,
pubmed-meshheading:1447360-Boranes,
pubmed-meshheading:1447360-Chromatography, Gas,
pubmed-meshheading:1447360-Chromatography, High Pressure Liquid,
pubmed-meshheading:1447360-Eggs,
pubmed-meshheading:1447360-Erythrocyte Membrane,
pubmed-meshheading:1447360-Female,
pubmed-meshheading:1447360-Glycerophosphates,
pubmed-meshheading:1447360-Hydrolysis,
pubmed-meshheading:1447360-Methanol,
pubmed-meshheading:1447360-Phospholipids,
pubmed-meshheading:1447360-Plasmalogens,
pubmed-meshheading:1447360-Rats,
pubmed-meshheading:1447360-Rats, Sprague-Dawley,
pubmed-meshheading:1447360-Sphingomyelins,
pubmed-meshheading:1447360-Sulfuric Acids
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pubmed:year |
1992
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pubmed:articleTitle |
Studies on the methanolysis of small amounts of purified phospholipids for gas chromatographic analysis of fatty acid methyl esters.
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pubmed:affiliation |
Institut für Ernährungsphysiologie, Technische Universität München,Freising-Weihenstephan, Germany.
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pubmed:publicationType |
Journal Article
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