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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
2
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pubmed:dateCreated |
1992-12-4
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pubmed:abstractText |
A recombinant soluble form (sEPO-R) of erythropoietin (EPO) receptor (EPO-R) was produced by Chinese hamster ovary cells and isolated in high yield with the EPO-fixed gel. Ligand binding assays were done using three methods; precipitation of sEPO-R radiolabeled EPO complex and competition of sEPO-R for the binding of radiolabeled EPO with the cellular EPO-R. The results showed a Kd of 17 nM which was much lower than those for cellular EPO-R. One N-glycosylation site exists in sEPO-R but the glycosylation did not affect the binding affinity to EPO. A complex with a molecular size that corresponded to a 1:1 complex of EPO and sEPO-R was detected.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
0006-291X
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:day |
30
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pubmed:volume |
188
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
888-97
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:1445329-Animals,
pubmed-meshheading:1445329-Base Sequence,
pubmed-meshheading:1445329-CHO Cells,
pubmed-meshheading:1445329-Cricetinae,
pubmed-meshheading:1445329-Erythropoietin,
pubmed-meshheading:1445329-Humans,
pubmed-meshheading:1445329-Introns,
pubmed-meshheading:1445329-Kinetics,
pubmed-meshheading:1445329-Molecular Sequence Data,
pubmed-meshheading:1445329-Oligodeoxyribonucleotides,
pubmed-meshheading:1445329-Polymerase Chain Reaction,
pubmed-meshheading:1445329-Receptors, Erythropoietin,
pubmed-meshheading:1445329-Recombinant Proteins,
pubmed-meshheading:1445329-Restriction Mapping,
pubmed-meshheading:1445329-Transfection
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pubmed:year |
1992
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pubmed:articleTitle |
Production and ligand-binding characteristics of the soluble form of murine erythropoietin receptor.
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pubmed:affiliation |
Department of Food Science and Technology, Faculty of Agriculture, Kyoto University, Japan.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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