Linked Life Data

1445241

Source:http://linkedlifedata.com/resource/pubmed/id/1445241

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:dateCreated
1992-12-11
pubmed:abstractText
In order to investigate the binding mode of E64-c (a synthetic cysteine proteinase inhibitor) to papain at the atomic level, the crystal structure of the complex was analysed by X-ray diffraction at 1.9 A (1 A is expressed in SI units as 0.1 nm) resolution. The crystal has a space group P2(1)2(1)2(1) with a = 43.37, b = 102.34 and c = 49.95 A. A total of 21,135 observed reflections were collected from the same crystal, and 14811 unique reflections of up to 1.9 A resolution [Fo > 3 sigma(Fo)] were used for the structure solution and refinement. The papain structure was determined by means of the molecular replacement method, and then the inhibitor was observed on a (2 magnitude of Fo-magnitude of Fc) difference Fourier map. The complex structure was finally refined to R = 19.4% including 207 solvent molecules. Although this complex crystal (Form II) was polymorphous as compared with the previously analysed one (Form I), the binding modes of leucine and isoamylamide moieties of E64-c were significantly different from each other. By the calculation of accessible surface area for each complex atom, these two different binding modes were both shown to be tight enough to prevent the access of solvent molecules to the papain active site. With respect to the E64-c-papain binding mode, molecular-dynamics simulations proposed two kinds of stationary states which were derived from the crystal structures of Forms I and II. One of these, which corresponds to the binding mode simulated from Form I, was essentially the same as that observed in the crystal structure, and the other was somewhat different from the crystal structure of Form II, especially with respect to the binding of the isoamylamide moiety with the papain S subsites. The substrate specificity for the papain active site is discussed on the basis of the present results.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/1445241-1130751, http://linkedlifedata.com/resource/pubmed/commentcorrection/1445241-1860874, http://linkedlifedata.com/resource/pubmed/commentcorrection/1445241-2700097, http://linkedlifedata.com/resource/pubmed/commentcorrection/1445241-2713367, http://linkedlifedata.com/resource/pubmed/commentcorrection/1445241-2924921, http://linkedlifedata.com/resource/pubmed/commentcorrection/1445241-3306875, http://linkedlifedata.com/resource/pubmed/commentcorrection/1445241-3438085, http://linkedlifedata.com/resource/pubmed/commentcorrection/1445241-4399049, http://linkedlifedata.com/resource/pubmed/commentcorrection/1445241-4946704, http://linkedlifedata.com/resource/pubmed/commentcorrection/1445241-5551392, http://linkedlifedata.com/resource/pubmed/commentcorrection/1445241-5884016, http://linkedlifedata.com/resource/pubmed/commentcorrection/1445241-6035483, http://linkedlifedata.com/resource/pubmed/commentcorrection/1445241-6393977, http://linkedlifedata.com/resource/pubmed/commentcorrection/1445241-6502713, http://linkedlifedata.com/resource/pubmed/commentcorrection/1445241-7044372, http://linkedlifedata.com/resource/pubmed/commentcorrection/1445241-952885
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
0264-6021
pubmed:author
pubmed:issnType
Print
pubmed:day
1
pubmed:volume
287 ( Pt 3)
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
797-803
pubmed:dateRevised
2010-9-7
pubmed:meshHeading
pubmed:year
1992
pubmed:articleTitle
Crystal structure of papain-E64-c complex. Binding diversity of E64-c to papain S2 and S3 subsites.
pubmed:affiliation
Department of Physical Chemistry, Osaka University of Pharmaceutical Sciences, Japan.
pubmed:publicationType
Journal Article