Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:dateCreated
1992-12-1
pubmed:abstractText
In this paper, we demonstrate the synthesis of the complement component C2 and factors B and D by the human astroglioma cell line U105-MG. All three components were structurally and antigenically similar to their serum counterparts, as determined by biosynthetic labelling studies or Western blot analysis. Northern blot analysis demonstrated that the mRNAs of all three components had the same apparent sizes as the equivalent mRNAs from hepatocyte and monocyte cell lines. Interestingly, U105-MG cells produce two C2 transcripts with sizes of approximately 2.8 and 2.3 kb. Interferon-gamma (IFN-gamma) enhanced the expression of C2 and factor B mRNA and protein in a dose- and time-dependent fashion, while factor D expression was refractory to IFN-gamma. IFN-gamma appeared to predominantly enhance the expression of the large (2.8 kb) C2 transcript. Kinetic studies demonstrated peak C2 and factor B expression in 48 h in response to IFN-gamma, similar to the acute-phase response of factor B in serum. These data are the first to demonstrate the synthesis of C2 and factor D by astroglioma cells. Combined with previous reports documenting the synthesis of C3 by astrocytes, our data suggest that endogenous synthesis of complement proteins, and particularly of alternative pathway activation components (C3, factors B and D), may play an important role in host defence in the central nervous system.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/1445220-1567547, http://linkedlifedata.com/resource/pubmed/commentcorrection/1445220-1701385, http://linkedlifedata.com/resource/pubmed/commentcorrection/1445220-1985940, http://linkedlifedata.com/resource/pubmed/commentcorrection/1445220-2121799, http://linkedlifedata.com/resource/pubmed/commentcorrection/1445220-2229060, http://linkedlifedata.com/resource/pubmed/commentcorrection/1445220-2324087, http://linkedlifedata.com/resource/pubmed/commentcorrection/1445220-2439072, http://linkedlifedata.com/resource/pubmed/commentcorrection/1445220-2492049, http://linkedlifedata.com/resource/pubmed/commentcorrection/1445220-2492582, http://linkedlifedata.com/resource/pubmed/commentcorrection/1445220-2493504, http://linkedlifedata.com/resource/pubmed/commentcorrection/1445220-2706954, http://linkedlifedata.com/resource/pubmed/commentcorrection/1445220-2866182, http://linkedlifedata.com/resource/pubmed/commentcorrection/1445220-3024123, http://linkedlifedata.com/resource/pubmed/commentcorrection/1445220-3088114, http://linkedlifedata.com/resource/pubmed/commentcorrection/1445220-3121777, http://linkedlifedata.com/resource/pubmed/commentcorrection/1445220-3142487, http://linkedlifedata.com/resource/pubmed/commentcorrection/1445220-3293952, http://linkedlifedata.com/resource/pubmed/commentcorrection/1445220-3299705, http://linkedlifedata.com/resource/pubmed/commentcorrection/1445220-3315100, http://linkedlifedata.com/resource/pubmed/commentcorrection/1445220-3492536, http://linkedlifedata.com/resource/pubmed/commentcorrection/1445220-3518744, http://linkedlifedata.com/resource/pubmed/commentcorrection/1445220-3655365, http://linkedlifedata.com/resource/pubmed/commentcorrection/1445220-3844050, http://linkedlifedata.com/resource/pubmed/commentcorrection/1445220-3881526, http://linkedlifedata.com/resource/pubmed/commentcorrection/1445220-388439, http://linkedlifedata.com/resource/pubmed/commentcorrection/1445220-3901003, http://linkedlifedata.com/resource/pubmed/commentcorrection/1445220-4559710, http://linkedlifedata.com/resource/pubmed/commentcorrection/1445220-518835, http://linkedlifedata.com/resource/pubmed/commentcorrection/1445220-6088497, http://linkedlifedata.com/resource/pubmed/commentcorrection/1445220-6199794, http://linkedlifedata.com/resource/pubmed/commentcorrection/1445220-6260907, http://linkedlifedata.com/resource/pubmed/commentcorrection/1445220-6312838, http://linkedlifedata.com/resource/pubmed/commentcorrection/1445220-6444659, http://linkedlifedata.com/resource/pubmed/commentcorrection/1445220-6559257, http://linkedlifedata.com/resource/pubmed/commentcorrection/1445220-6561229, http://linkedlifedata.com/resource/pubmed/commentcorrection/1445220-82604
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0264-6021
pubmed:author
pubmed:issnType
Print
pubmed:day
15
pubmed:volume
287 ( Pt 2)
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
595-601
pubmed:dateRevised
2011-11-17
pubmed:meshHeading
pubmed:year
1992
pubmed:articleTitle
Production and interferon-gamma-mediated regulation of complement component C2 and factors B and D by the astroglioma cell line U105-MG.
pubmed:affiliation
Department of Microbiology, University of Alabama, Birmingham 35294.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't