| pubmed-article:1444414 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:1444414 | lifeskim:mentions | umls-concept:C0237401 | lld:lifeskim |
| pubmed-article:1444414 | lifeskim:mentions | umls-concept:C0020792 | lld:lifeskim |
| pubmed-article:1444414 | lifeskim:mentions | umls-concept:C0686817 | lld:lifeskim |
| pubmed-article:1444414 | lifeskim:mentions | umls-concept:C0028952 | lld:lifeskim |
| pubmed-article:1444414 | pubmed:issue | 9 | lld:pubmed |
| pubmed-article:1444414 | pubmed:dateCreated | 1992-12-23 | lld:pubmed |
| pubmed-article:1444414 | pubmed:abstractText | A method to microscopically detect and identify individual cells of members of the domains Bacteria and Archaea is presented. rRNA-targeted oligonucleotides were 5' end labeled with the enzyme horseradish peroxidase and used for whole-cell hybridization. Specifically bound probe was visualized by the enzymatic formation of an intracellular precipitate from the substrate diaminobenzidine. Permeation of the enzyme-labeled probe into whole fixed cells of gram-negative bacteria required their pretreatment with lysozyme-EDTA, whereas permeability of some archaebacterial cells was improved by addition of detergent to the hybridization buffer. Hitherto we had not achieved penetration of enzyme-labeled probe into gram-positive bacteria and yeast cells. This method should be a valuable tool for identification of suitable prokaryotic cells in environments with elevated background fluorescence or in situations in which an epifluorescence microscope is not available. | lld:pubmed |
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| pubmed-article:1444414 | pubmed:language | eng | lld:pubmed |
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| pubmed-article:1444414 | pubmed:citationSubset | IM | lld:pubmed |
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| pubmed-article:1444414 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:1444414 | pubmed:month | Sep | lld:pubmed |
| pubmed-article:1444414 | pubmed:issn | 0099-2240 | lld:pubmed |
| pubmed-article:1444414 | pubmed:author | pubmed-author:SchleiferK... | lld:pubmed |
| pubmed-article:1444414 | pubmed:author | pubmed-author:StahlD ADA | lld:pubmed |
| pubmed-article:1444414 | pubmed:author | pubmed-author:AmannR IRI | lld:pubmed |
| pubmed-article:1444414 | pubmed:author | pubmed-author:ZardaBB | lld:pubmed |
| pubmed-article:1444414 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:1444414 | pubmed:volume | 58 | lld:pubmed |
| pubmed-article:1444414 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:1444414 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:1444414 | pubmed:pagination | 3007-11 | lld:pubmed |
| pubmed-article:1444414 | pubmed:dateRevised | 2010-9-7 | lld:pubmed |
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| pubmed-article:1444414 | pubmed:year | 1992 | lld:pubmed |
| pubmed-article:1444414 | pubmed:articleTitle | Identification of individual prokaryotic cells by using enzyme-labeled, rRNA-targeted oligonucleotide probes. | lld:pubmed |
| pubmed-article:1444414 | pubmed:affiliation | Lehrstuhl für Mikrobiologie, Technische Universität München, Germany. | lld:pubmed |
| pubmed-article:1444414 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:1444414 | pubmed:publicationType | Research Support, U.S. Gov't, Non-P.H.S. | lld:pubmed |
| pubmed-article:1444414 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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