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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
9
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pubmed:dateCreated |
1992-12-23
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pubmed:abstractText |
A method to microscopically detect and identify individual cells of members of the domains Bacteria and Archaea is presented. rRNA-targeted oligonucleotides were 5' end labeled with the enzyme horseradish peroxidase and used for whole-cell hybridization. Specifically bound probe was visualized by the enzymatic formation of an intracellular precipitate from the substrate diaminobenzidine. Permeation of the enzyme-labeled probe into whole fixed cells of gram-negative bacteria required their pretreatment with lysozyme-EDTA, whereas permeability of some archaebacterial cells was improved by addition of detergent to the hybridization buffer. Hitherto we had not achieved penetration of enzyme-labeled probe into gram-positive bacteria and yeast cells. This method should be a valuable tool for identification of suitable prokaryotic cells in environments with elevated background fluorescence or in situations in which an epifluorescence microscope is not available.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/1444414-103504,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1444414-13611202,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1444414-1688842,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1444414-1709451,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1444414-1724265,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1444414-1967116,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1444414-2112744,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1444414-2200342,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1444414-2466341,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1444414-2494460,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1444414-3299267,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1444414-3387214,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1444414-3519544,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1444414-3748805,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1444414-5962951,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1444414-7061820
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Sep
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pubmed:issn |
0099-2240
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
58
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
3007-11
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pubmed:dateRevised |
2010-9-7
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pubmed:meshHeading |
pubmed-meshheading:1444414-Archaea,
pubmed-meshheading:1444414-Bacteria,
pubmed-meshheading:1444414-Base Sequence,
pubmed-meshheading:1444414-Cell Membrane Permeability,
pubmed-meshheading:1444414-Horseradish Peroxidase,
pubmed-meshheading:1444414-Molecular Sequence Data,
pubmed-meshheading:1444414-Nucleic Acid Hybridization,
pubmed-meshheading:1444414-Oligonucleotide Probes,
pubmed-meshheading:1444414-Prokaryotic Cells,
pubmed-meshheading:1444414-RNA, Ribosomal,
pubmed-meshheading:1444414-RNA Probes
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pubmed:year |
1992
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pubmed:articleTitle |
Identification of individual prokaryotic cells by using enzyme-labeled, rRNA-targeted oligonucleotide probes.
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pubmed:affiliation |
Lehrstuhl für Mikrobiologie, Technische Universität München, Germany.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, Non-P.H.S.,
Research Support, Non-U.S. Gov't
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