Linked Life Data

1443097

Source:http://linkedlifedata.com/resource/pubmed/id/1443097

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5 Pt 1
pubmed:dateCreated
1992-12-23
pubmed:abstractText
Myocardial synthesis of phosphatidylinositol 4,5-bisphosphate (PIP2) is highly compartmentalized in the sarcolemmal membrane. Sarcolemmal vesicles contain endogenous phospholipase C (PLC), but the identity of sarcolemmal PLC and its relationship to soluble PLC have not been determined previously. Sarcolemmal and cytosolic PLC were prepared from canine myocardium and characterized by DEAE-cellulose chromatography and by immunoblotting with monoclonal and polyclonal antibodies to isoenzymes of PLC (PLC beta, PLC gamma, and PLC delta). DEAE-cellulose chromatography resolved two forms of cytosolic PLC that were identified as an 85-kDa form of PLC delta and a 145-kDa form of PLC gamma. In contrast, DEAE-cellulose chromatography resolved a single form of sarcolemmal PLC that was identified as an 85-kDa form of PLC delta. These data demonstrate that PLC gamma and PLC delta are expressed in canine myocardium and that an 85-kDa form of PLC delta is selectively associated with sites of PIP2 synthesis in a highly enriched preparation of sarcolemma. These data do not exclude the existence of additional isoenzymes of sarcolemmal PLC that may have been removed during isolation of sarcolemmal membranes.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
0002-9513
pubmed:author
pubmed:issnType
Print
pubmed:volume
263
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
C1021-8
pubmed:dateRevised
2007-11-15
pubmed:meshHeading
pubmed:year
1992
pubmed:articleTitle
Association of phospholipase C-delta with a highly enriched preparation of canine sarcolemma.
pubmed:affiliation
Department of Medicine, Washington University School of Medicine, St. Louis, Missouri 63110.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't