rdf:type |
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lifeskim:mentions |
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pubmed:dateCreated |
1965-12-1
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pubmed:abstractText |
1. Extracts of Landschutz ascites-tumour cells have been fractionated by treatment with acid, alumina C(gamma) gel and Sephadex G-100 to yield purified preparations of thymidine phosphokinase, thymidine 5'-monophosphate phosphokinase and thymidine 5'-diphosphate phosphokinase. 2. These results clearly demonstrate the existence in Landschutz ascites tumour of three phosphokinases each of which catalyses one step in the reaction sequence: thymidineright harpoon over left harpoonthymidine 5'-monophosphateright harpoon over left harpoonthymidine 5'-diphosphateright harpoon over left harpoonthymidine 5'-triphosphate. Though these results do not preclude the participation of other enzymes in the formation of thymidine 5'-triphosphate from thymidine by Landschutz ascites-tumour cells, they provide strong support for the view that thymidine 5'-diphosphate is an intermediate in the formation of thymidine 5'-triphosphate from thymidine 5'-monophosphate by this system.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/14340042-13784139,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14340042-13835290,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14340042-13868896,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14340042-13964236,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14340042-13967158,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14340042-14015204,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14340042-14101968,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14340042-14114853,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14340042-14405178,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14340042-14405566,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14340042-14907713
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pubmed:keyword |
http://linkedlifedata.com/resource/pubmed/keyword/BIOCHEMISTRY,
http://linkedlifedata.com/resource/pubmed/keyword/CHEMISTRY, ANALYTICAL,
http://linkedlifedata.com/resource/pubmed/keyword/CHROMATOGRAPHY,
http://linkedlifedata.com/resource/pubmed/keyword/DEXTRAN,
http://linkedlifedata.com/resource/pubmed/keyword/EXPERIMENTAL LAB STUDY,
http://linkedlifedata.com/resource/pubmed/keyword/GEL FILTRATION,
http://linkedlifedata.com/resource/pubmed/keyword/NUCLEOTIDES,
http://linkedlifedata.com/resource/pubmed/keyword/SPECTROPHOTOMETRY,
http://linkedlifedata.com/resource/pubmed/keyword/THYMIDINE KINASE,
http://linkedlifedata.com/resource/pubmed/keyword/TISSUE CULTURE
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
OM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Mar
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pubmed:issn |
0264-6021
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
94
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
518-24
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pubmed:dateRevised |
2011-9-28
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pubmed:meshHeading |
pubmed-meshheading:14340042-Biochemical Phenomena,
pubmed-meshheading:14340042-Biochemistry,
pubmed-meshheading:14340042-Chemistry Techniques, Analytical,
pubmed-meshheading:14340042-Chromatography,
pubmed-meshheading:14340042-Chromatography, Gel,
pubmed-meshheading:14340042-Dextrans,
pubmed-meshheading:14340042-Nucleotides,
pubmed-meshheading:14340042-Research,
pubmed-meshheading:14340042-Spectrophotometry,
pubmed-meshheading:14340042-Thymidine Kinase,
pubmed-meshheading:14340042-Tissue Culture Techniques
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pubmed:year |
1965
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pubmed:articleTitle |
FRACTIONATION OF THYMIDINE PHOSPHOKINASE, THYMIDINE 5'-MONOPHOSPHATE PHOSPHOKINASE AND THYMIDINE 5'-DIPHOSPHATE PHOSPHOKINASE IN EXTRACTS OF LANDSCHUTZ ASCITES-TUMOUR CELLS.
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pubmed:publicationType |
Journal Article
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