Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:dateCreated
1965-11-1
pubmed:abstractText
1. A method is described for preparing pure samples of 19s gamma-globulin (IgM) from normal human serum by using successive steps of dialysis, density-gradient ultracentrifugation, chromatography on DEAE-cellulose, and gel filtration on Sephadex G-200. The yield of IgM (20-25mg./100ml. of serum) was equivalent to about one-quarter of that present in normal serum. 2. Analysis of the separated peptide chains of normal IgM and IgG (7s gamma-globulin) showed considerable differences in the amino acid composition of A chains from the two proteins; their respective B chains, on the other hand, were similar in composition. The carbohydrate of both proteins is confined almost entirely to the A chains; the IgM A chain contains about four times as much carbohydrate as the IgG A chain. 3. These findings support the view that the different classes of human immunoglobulin have B chains that are identical and A chains that are chemically distinct.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/14335812-13262900, http://linkedlifedata.com/resource/pubmed/commentcorrection/14335812-13370604, http://linkedlifedata.com/resource/pubmed/commentcorrection/14335812-13539040, http://linkedlifedata.com/resource/pubmed/commentcorrection/14335812-13619782, http://linkedlifedata.com/resource/pubmed/commentcorrection/14335812-13639270, http://linkedlifedata.com/resource/pubmed/commentcorrection/14335812-13672998, http://linkedlifedata.com/resource/pubmed/commentcorrection/14335812-13725659, http://linkedlifedata.com/resource/pubmed/commentcorrection/14335812-13743526, http://linkedlifedata.com/resource/pubmed/commentcorrection/14335812-13790184, http://linkedlifedata.com/resource/pubmed/commentcorrection/14335812-13915219, http://linkedlifedata.com/resource/pubmed/commentcorrection/14335812-13945475, http://linkedlifedata.com/resource/pubmed/commentcorrection/14335812-14018549, http://linkedlifedata.com/resource/pubmed/commentcorrection/14335812-14022096, http://linkedlifedata.com/resource/pubmed/commentcorrection/14335812-14039073, http://linkedlifedata.com/resource/pubmed/commentcorrection/14335812-14063859, http://linkedlifedata.com/resource/pubmed/commentcorrection/14335812-14063860, http://linkedlifedata.com/resource/pubmed/commentcorrection/14335812-14068229, http://linkedlifedata.com/resource/pubmed/commentcorrection/14335812-14082032, http://linkedlifedata.com/resource/pubmed/commentcorrection/14335812-14084620, http://linkedlifedata.com/resource/pubmed/commentcorrection/14335812-14089762, http://linkedlifedata.com/resource/pubmed/commentcorrection/14335812-14114610, http://linkedlifedata.com/resource/pubmed/commentcorrection/14335812-14166585, http://linkedlifedata.com/resource/pubmed/commentcorrection/14335812-14167907, http://linkedlifedata.com/resource/pubmed/commentcorrection/14335812-14393571, http://linkedlifedata.com/resource/pubmed/commentcorrection/14335812-16747652, http://linkedlifedata.com/resource/pubmed/commentcorrection/14335812-4157898, http://linkedlifedata.com/resource/pubmed/commentcorrection/14335812-4158025, http://linkedlifedata.com/resource/pubmed/commentcorrection/14335812-4378798
pubmed:keyword
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
OM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0264-6021
pubmed:author
pubmed:issnType
Print
pubmed:volume
95
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
256-61
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1965
pubmed:articleTitle
PREPARATION AND PROPERTIES OF THE PEPTIDE CHAINS OF NORMAL HUMAN 19 S GAMMA-GLOBULIN (IGM).
pubmed:publicationType
Journal Article