Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:dateCreated
1965-11-1
pubmed:abstractText
Holmes, P. K. (University of Illinois, Urbana), and H. O. Halvorson. Purification of a salt-requiring enzyme from an obligately halophilic bacterium. J. Bacteriol. 90: 312-315. 1965.-The discovery that some halophilic enzymes may be reactivated from the salt-free and inactive state has facilitated the purification of nicotinamide adenine dinucleotide-linked malic acid dehydrogenase from crude extracts of Halobacterium salinarium. In the absence of large amounts of salt, the enzyme was totally inactive; yet, in this salt-free state, it could be purified by conventional techniques. The highly purified halophilic enzyme was shown to require a high concentration of salt for activity.
pubmed:commentsCorrections
pubmed:keyword
http://linkedlifedata.com/resource/pubmed/keyword/ACETONE, http://linkedlifedata.com/resource/pubmed/keyword/CHEMISTRY, http://linkedlifedata.com/resource/pubmed/keyword/CHEMISTRY, ANALYTICAL, http://linkedlifedata.com/resource/pubmed/keyword/CHROMATOGRAPHY, http://linkedlifedata.com/resource/pubmed/keyword/DIALYSIS, http://linkedlifedata.com/resource/pubmed/keyword/ELECTROPHORESIS, http://linkedlifedata.com/resource/pubmed/keyword/EXPERIMENTAL LAB STUDY, http://linkedlifedata.com/resource/pubmed/keyword/HALOBACTERIUM, http://linkedlifedata.com/resource/pubmed/keyword/HYDROGEN-ION CONCENTRATION, http://linkedlifedata.com/resource/pubmed/keyword/MALATE DEHYDROGENASE, http://linkedlifedata.com/resource/pubmed/keyword/METABOLISM, http://linkedlifedata.com/resource/pubmed/keyword/NAD, http://linkedlifedata.com/resource/pubmed/keyword/SODIUM CHLORIDE
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
OM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
0021-9193
pubmed:author
pubmed:issnType
Print
pubmed:volume
90
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
312-5
pubmed:dateRevised
2009-11-19
pubmed:meshHeading
pubmed:year
1965
pubmed:articleTitle
PURIFICATION OF A SALT-REQUIRING ENZYME FROM AN OBLIGATELY HALOPHILIC BACTERIUM.
pubmed:publicationType
Journal Article