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pubmed-article:1432363pubmed:abstractTextIn order to study the concentration of pancreatic stone protein (PSP) in human pancreatic juice, we investigated the influence of the insoluble form of PSP-S1 converted from PSP-S2-5 on PSP determination and the assay method for PSP-S1 precipitate after solubilizing PSP-S1. When bovine trypsin was added to pancreatic juice, PSP-S1 was converted from PSP-S2-5 and precipitated about 45-85% after 1 h. The precipitated PSP-S1 was dissolved in 0.1 M sodium acetate buffer, pH 4.0, and the concentration was measured by the enzyme immunoassay, with similar reactivity to PSP-S1 and PSP-S2-5. The proposed method can offer accurate and specific analysis of the PSP level in pancreatic juice. The results of the fractionation of pancreatic juice and duodenal juice on Mono S cation-exchange chromatography suggested that the major component of PSP was PSP-S2-5 in pancreatic juice and PSP-S1 in duodenal juice.lld:pubmed
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pubmed-article:1432363pubmed:articleTitleEnzyme immunoassay for specific analysis of pancreatic stone proteins in human pancreatic juice.lld:pubmed
pubmed-article:1432363pubmed:affiliationDepartment of Clinical Chemistry, Maruko Pharmaceutical Co., Ltd., Kasugai, Japan.lld:pubmed
pubmed-article:1432363pubmed:publicationTypeJournal Articlelld:pubmed
pubmed-article:1432363pubmed:publicationTypeResearch Support, Non-U.S. Gov'tlld:pubmed