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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
6
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pubmed:dateCreated |
1992-12-22
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pubmed:abstractText |
In order to study the concentration of pancreatic stone protein (PSP) in human pancreatic juice, we investigated the influence of the insoluble form of PSP-S1 converted from PSP-S2-5 on PSP determination and the assay method for PSP-S1 precipitate after solubilizing PSP-S1. When bovine trypsin was added to pancreatic juice, PSP-S1 was converted from PSP-S2-5 and precipitated about 45-85% after 1 h. The precipitated PSP-S1 was dissolved in 0.1 M sodium acetate buffer, pH 4.0, and the concentration was measured by the enzyme immunoassay, with similar reactivity to PSP-S1 and PSP-S2-5. The proposed method can offer accurate and specific analysis of the PSP level in pancreatic juice. The results of the fractionation of pancreatic juice and duodenal juice on Mono S cation-exchange chromatography suggested that the major component of PSP was PSP-S2-5 in pancreatic juice and PSP-S1 in duodenal juice.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:issn |
0887-8013
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
6
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
375-8
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pubmed:dateRevised |
2007-11-15
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pubmed:meshHeading |
pubmed-meshheading:1432363-Calcium-Binding Proteins,
pubmed-meshheading:1432363-Chromatography, High Pressure Liquid,
pubmed-meshheading:1432363-Chronic Disease,
pubmed-meshheading:1432363-Duodenum,
pubmed-meshheading:1432363-Evaluation Studies as Topic,
pubmed-meshheading:1432363-Humans,
pubmed-meshheading:1432363-Immunoenzyme Techniques,
pubmed-meshheading:1432363-Intestinal Secretions,
pubmed-meshheading:1432363-Lithostathine,
pubmed-meshheading:1432363-Nerve Tissue Proteins,
pubmed-meshheading:1432363-Pancreatic Juice,
pubmed-meshheading:1432363-Pancreatitis,
pubmed-meshheading:1432363-Reference Values,
pubmed-meshheading:1432363-Solubility,
pubmed-meshheading:1432363-Trypsin
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pubmed:year |
1992
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pubmed:articleTitle |
Enzyme immunoassay for specific analysis of pancreatic stone proteins in human pancreatic juice.
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pubmed:affiliation |
Department of Clinical Chemistry, Maruko Pharmaceutical Co., Ltd., Kasugai, Japan.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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