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rdf:type |
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lifeskim:mentions |
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pubmed:dateCreated |
1992-12-10
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pubmed:databankReference |
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pubmed:abstractText |
The complete RNA genome of turnip mosaic potyvirus (TuMV) was amplified by seven consecutive reverse transcriptase-polymerase chain reactions and cloned into pUC9. The viral RNA is 9830 nucleotides long and contains a single open reading frame (ORF) of 9489 bases encoding a large polyprotein of 3863 amino acids with a calculated M(r) of 358,000. The non-coding region (NCR) preceding the ORF is 129 nucleotides long and has a high AU content (70%). Its predicted secondary structure is characterized by a hairpin loop with a free energy loss of -69.9 kJ/mol. The termination codon is followed by an AU-rich NCR of 209 bases, excluding the poly(A) tail. Seven potential nuclear inclusion a proteinase (NIa-Pro) recognition heptapeptides are found in the polyprotein. Their sequences agree with consensus potyviral NIa-Pro cleavage sequences except for that at the 6K-VPg site, which is characterized by a glutamic acid residue preceding the hydrolysed peptide bond. The TuMV proteins are similar to their corresponding potyviral proteins.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Nov
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pubmed:issn |
0022-1317
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
73 ( Pt 11)
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
2785-93
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pubmed:dateRevised |
2007-10-11
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pubmed:meshHeading |
pubmed-meshheading:1431807-Amino Acid Sequence,
pubmed-meshheading:1431807-Base Sequence,
pubmed-meshheading:1431807-Brassica,
pubmed-meshheading:1431807-Chromosome Mapping,
pubmed-meshheading:1431807-Cloning, Molecular,
pubmed-meshheading:1431807-Consensus Sequence,
pubmed-meshheading:1431807-Endopeptidases,
pubmed-meshheading:1431807-Genome, Viral,
pubmed-meshheading:1431807-Molecular Sequence Data,
pubmed-meshheading:1431807-Mosaic Viruses,
pubmed-meshheading:1431807-Nucleic Acid Conformation,
pubmed-meshheading:1431807-Open Reading Frames,
pubmed-meshheading:1431807-Protein Processing, Post-Translational,
pubmed-meshheading:1431807-RNA, Viral,
pubmed-meshheading:1431807-Sequence Homology, Amino Acid,
pubmed-meshheading:1431807-Viral Proteins
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pubmed:year |
1992
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pubmed:articleTitle |
The complete nucleotide sequence of turnip mosaic potyvirus RNA.
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pubmed:affiliation |
Centre de Recherche en Virologie, Institut Armand-Frappier, Ville de Laval, Québec, Canada.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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