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rdf:type |
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lifeskim:mentions |
umls-concept:C0003241,
umls-concept:C0017262,
umls-concept:C0033621,
umls-concept:C0034493,
umls-concept:C0205171,
umls-concept:C0205369,
umls-concept:C0686817,
umls-concept:C1171362,
umls-concept:C1514562,
umls-concept:C1515670,
umls-concept:C1880389,
umls-concept:C1883204,
umls-concept:C1883221
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pubmed:issue |
1
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pubmed:dateCreated |
1992-12-4
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pubmed:abstractText |
VDJ genes were amplified by the polymerase chain reaction from mRNA isolated from peripheral blood B cells of rabbits immunized with protein C. The amplified genes were cloned into a lambda phage expression vector and packaged. A library of 6 x 10(5) recombinant phages was screened with labelled protein C and 30 positive clones were found. Three of them were plaque purified and the affinity of the single domain antibodies to the antigen determined to be 10(6)-10(7) l M-1. The data indicate the feasibility of generating single domain antibody, specific to protein antigen, from rabbit.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
0165-2478
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
33
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
53-9
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:1427991-Animals,
pubmed-meshheading:1427991-Antibodies,
pubmed-meshheading:1427991-Antibody Specificity,
pubmed-meshheading:1427991-Base Sequence,
pubmed-meshheading:1427991-Escherichia coli,
pubmed-meshheading:1427991-Feasibility Studies,
pubmed-meshheading:1427991-Gene Amplification,
pubmed-meshheading:1427991-Genes, Immunoglobulin,
pubmed-meshheading:1427991-Immunoglobulin J-Chains,
pubmed-meshheading:1427991-Immunoglobulin Variable Region,
pubmed-meshheading:1427991-Immunoglobulin delta-Chains,
pubmed-meshheading:1427991-Molecular Sequence Data,
pubmed-meshheading:1427991-Polymerase Chain Reaction,
pubmed-meshheading:1427991-Protein C,
pubmed-meshheading:1427991-Rabbits,
pubmed-meshheading:1427991-Recombinant Fusion Proteins,
pubmed-meshheading:1427991-Species Specificity
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pubmed:year |
1992
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pubmed:articleTitle |
Rabbit single domain antibodies specific to protein C expressed in prokaryotes.
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pubmed:affiliation |
Schweizerisches Institut für Allergie- und Asthmaforschung, Davos, Switzerland.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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