Linked Life Data

14276107

Source:http://linkedlifedata.com/resource/pubmed/id/14276107

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:dateCreated
1965-7-1
pubmed:abstractText
Chatterjee, B. R. (Baylor University College of Medicine, Houston, Tex.), and Robert P. Williams. Formation of spheroplasts from Bacillus anthracis. J. Bacteriol. 89:1128-1133. 1965.-Spheroplasts were prepared from Bacillus anthracis by combined treatment with lysozyme and glycine. Glycine, at a final concentration of 3%, was added to cultures of B. anthracis in nutrient broth that had grown at 37 C for 16 to 18 hr under 50% CO(2). After additional incubation under CO(2) for 2 hr, lysozyme, at the appropriate concentration (50 to 100 mug/ml), and sucrose, to a concentration of 15%, were added, and incubation was continued for 2 to 6 hr in CO(2). At the end of this period, incubation in CO(2) was discontinued. Spheroplasts formed after incubation in air for 6 to 12 hr. Lysozyme alone exhibited the same effect when added at much higher concentrations (500 to 2,000 mug/ml) to cultures growing under CO(2). No spheroplasts formed when cultures were treated with glycine alone. Treatment with lysozyme was more effective on smooth strains than rough. Cells from young cultures were more susceptible to lysozyme than older cells. CO(2) apparently was essential for formation of spheroplasts from B. anthracis.
pubmed:commentsCorrections
pubmed:keyword
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
OM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0021-9193
pubmed:author
pubmed:issnType
Print
pubmed:volume
89
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1128-33
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1965
pubmed:articleTitle
FORMATION OF SPHEROPLASTS FROM BACILLUS ANTHRACIS.
pubmed:publicationType
Journal Article